Olsnes S, Tønnessen T I, Ludt J, Sandvig K
Biochemistry. 1987 May 19;26(10):2778-85. doi: 10.1021/bi00384a019.
The effect of the internal pH on the rate of 36Cl- uptake and efflux was measured. While the rate of 36Cl- uptake by the antiport increased almost 10-fold over a narrow pH range in a number of cell lines, in other cells the pH-induced increase was considerably less. With increasing pH, the rate of 36Cl- efflux into chloride-free buffers increased strongly over a narrow pH range in all cell lines studied. Two groups of cell lines appeared, one group where the half-maximal increase in uptake and efflux rate was at pH 7.0-7.1 and another group where the corresponding values were 0.2-0.4 pH unit higher. The stoichiometry between chloride influx and chloride-stimulated efflux was close to 1:1 under various conditions.
测定了细胞内pH对³⁶Cl⁻摄取和流出速率的影响。在许多细胞系中，反向转运体对³⁶Cl⁻的摄取速率在狭窄的pH范围内几乎增加了10倍，而在其他细胞中，pH诱导的增加则要少得多。随着pH升高，在所有研究的细胞系中，³⁶Cl⁻向无氯缓冲液中的流出速率在狭窄的pH范围内急剧增加。出现了两组细胞系，一组摄取和流出速率增加到最大值一半时的pH为7.0 - 7.1，另一组相应的值高0.2 - 0.4个pH单位。在各种条件下，氯离子流入与氯离子刺激流出之间的化学计量比接近1:1。
