Olsnes S, Tønnessen T I, Sandvig K
J Cell Biol. 1986 Mar;102(3):967-71. doi: 10.1083/jcb.102.3.967.
The uptake of 36Cl- into cells was measured after preincubation in medium containing nigericin and KCl to allow control of the intracellular pH. When the pH was increased from pH 7.0 to pH 7.3 there was a 10-fold increase in the rate of 36Cl- uptake. The increase was half maximal at pH 7.15 in Vero and L-cells, whereas in phorbol 12-myristate 13-acetate-treated Vero cells the increase was half maximal at pH 6.9. Kinetic studies showed that in cells preincubated with nigericin and isotonic KCl, both at pH 7.0 and at pH 8.0, the Km for Cl- was 7 mM. In the two cases the Jmax was 1.7 X 10(8) Cl- ions X cell-1 X s-1 and 1.6 X 10(9) Cl- ions X cell-1 X s-1, respectively. Bicarbonate inhibited 36Cl- uptake with a Ki of 5-6 mM. Probably, the anion antiporter plays a role in the regulation of the intracellular pH.
在含有尼日利亚菌素和氯化钾的培养基中预孵育细胞后，测量细胞对³⁶Cl⁻的摄取，以控制细胞内pH值。当pH值从7.0增加到7.3时，³⁶Cl⁻摄取速率增加了10倍。在Vero细胞和L细胞中，pH值为7.15时增加量达到最大值的一半，而在用佛波醇12 -肉豆蔻酸酯13 -乙酸酯处理的Vero细胞中，pH值为6.9时增加量达到最大值的一半。动力学研究表明，在预先用尼日利亚菌素和等渗氯化钾孵育的细胞中，无论pH值为7.0还是8.0，Cl⁻的Km值均为7 mM。在这两种情况下，Jmax分别为1.7×10⁸个Cl⁻离子×细胞⁻¹×秒⁻¹和1.6×10⁹个Cl⁻离子×细胞⁻¹×秒⁻¹。碳酸氢盐以5 - 6 mM的Ki抑制³⁶Cl⁻摄取。阴离子反向转运体可能在细胞内pH值的调节中起作用。
