The Institute of Low Temperature Science, Hokkaido University, Kita-19, Nishi-8, Kita-ku, Sapporo, 060-0819, Japan.
Graduate School of Environmental Science, Hokkaido University, Kita-10, Nishi-5, Kita-ku, Sapporo, 060-0810, Japan.
Arch Microbiol. 2022 Sep 7;204(10):605. doi: 10.1007/s00203-022-03221-0.
A novel sulfur-oxidizing bacterium, strain Am19, was isolated from sediment of a brackish lake. Strain Am19 grew chemolithoautotrophically on inorganic sulfur compounds, and heterotrophic growth was not observed. Cells were rod-shaped with length of 1.1-3.0 μm and diameter of 0.5-0.8 μm. Growth was observed at 5-37 °C with an optimum growth temperature of 30 °C. The pH range for growth was 5.6-8.5 with an optimum pH of 6.6-7.0. Major fatty acids were summed feature 3 (Cω7c and/or Cω6c), summed feature 8 (Cω7c and/or Cω6c) and C. The sole respiratory quinone was ubiquinone-8. The complete genome of strain Am19 is composed of a circular chromosome with length of 2.5 Mbp and G + C content of 42.7 mol%. Phylogenetic analysis based on genomic data indicated that strain Am19 belongs to the genus Thiomicrorhabdus but is distinct from any existing species. Analysis of the 16S rRNA gene supported creation of a new species to accommodate strain Am19. On the basis of genomic and phenotypic characteristics, strain Am19 (= NBRC 114602 = BCRC 81336) is proposed as the type strain of a new species, with name of Thiomicrorhabdus immobilis sp. nov.
从一个半咸水湖泊的沉积物中分离到一株新型硫氧化细菌，菌株 Am19。该菌能够以无机硫化合物为唯一能源进行化能自养生长，不能进行异养生长。细胞呈杆状，大小为 1.1-3.0μm×0.5-0.8μm。在 5-37°C 范围内生长，最适生长温度为 30°C。生长的 pH 范围为 5.6-8.5，最适 pH 为 6.6-7.0。主要脂肪酸为 sumF3（Cω7c 和/或 Cω6c）、sumF8（Cω7c 和/或 Cω6c）和 C16:0。唯一的呼吸醌为泛醌-8。菌株 Am19 的全基因组由一条大小为 2.5 Mbp 的环状染色体和 42.7 mol%的 G+C 含量组成。基于基因组数据的系统发育分析表明，菌株 Am19 属于硫微螺菌属，但与任何现有种均不同。16S rRNA 基因分析支持创建一个新种以容纳菌株 Am19。基于基因组和表型特征，菌株 Am19（=NBRC 114602=BCRC 81336）被提议为一个新种的模式菌株，命名为不动硫微螺菌（Thiomicrorhabdus immobilis）sp. nov.。
