酸催化乙二醇-水预处理甘蔗渣以提高纤维素的酶转化。
Pretreatment of sugarcane bagasse with acid catalyzed ethylene glycol-water to improve the cellulose enzymatic conversion.
机构信息
Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, Nanjing Forestry University, No. 159 Longpan Road, Nanjing 210037, China; Joint International Research Lab of Lignocellulosic Functional Materials, Nanjing Forestry University, No. 159 Longpan Road, Nanjing 210037, China.
Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, Nanjing Forestry University, No. 159 Longpan Road, Nanjing 210037, China; Joint International Research Lab of Lignocellulosic Functional Materials, Nanjing Forestry University, No. 159 Longpan Road, Nanjing 210037, China.
出版信息
Bioresour Technol. 2022 Oct;361:127723. doi: 10.1016/j.biortech.2022.127723. Epub 2022 Jul 30.
In this work, HCl catalyzed ethylene glycol-water pretreatment (HCl/EG-HO) of sugarcane bagasse (SCB) was explored with response surface methodology (RSM) and single factor analysis, which aim to investigate the influence of pretreatment variable on pretreated solid cellulose enzymatic conversion. The result showed that HCl/EG-HO pretreatment could selectively extract ∼89.9 % xylan and ∼61.2 % lignin in SCB, meanwhile maintain a relatively high cellulose retention (∼86.8 %). Pretreatment of SCB at 120 °C for 60 min with 1.00 % HCl and 90 % EG obtained the pretreated solid having maximum cellulose enzymatic conversion of 88.7 % under 10 FPU/g enzyme dosage, this enhancement of cellulose enzymatic conversion mainly attributed to structure change of SCB in pretreatment. The adding of enzymatic additives into the hydrolysis process could not only improve hydrolysis efficiency but also lower the enzyme dosage. Besides, the linear relationship between substrate characteristic parameters (such cellulose content, lignin removal rate etc.) and cellulose conversion were observed.
在这项工作中，采用响应面法（RSM）和单因素分析探讨了 HCl 催化的乙二醇-水预处理（HCl/EG-HO）对甘蔗渣（SCB）的作用，旨在研究预处理变量对预处理固体纤维素酶转化的影响。结果表明，HCl/EG-HO 预处理可以选择性地提取 SCB 中约 89.9%的木聚糖和约 61.2%的木质素，同时保持相对较高的纤维素保留率（约 86.8%）。在 120°C 下用 1.00%HCl 和 90%EG 预处理 SCB60min，可在 10 FPU/g 酶用量下获得最大纤维素酶转化率为 88.7%的预处理固体，纤维素酶转化率的提高主要归因于预处理过程中 SCB 的结构变化。在水解过程中添加酶添加剂不仅可以提高水解效率，还可以降低酶用量。此外，还观察到了底物特征参数（如纤维素含量、木质素去除率等）与纤维素转化率之间的线性关系。
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