Cellular Therapy and Stem Cell Production Application, Research Centre (ESTEM), Institute of Health Sciences, Eskisehir Osmangazi University, 26040, Eskisehir, Turkey.
Department of Stem Cell, Institute of Health Sciences, Eskisehir Osmangazi University, Eskisehir, Turkey.
Med Oncol. 2022 Sep 7;39(12):191. doi: 10.1007/s12032-022-01793-x.
Klotho is an anti-aging, anti-inflammator, and anti-oxidative protein and has been shown to important role in tumorigenesis, proliferation, survival, autophagy, and resistance to tumor suppressor effects in several types of human cancers. In this study, we aimed to investigate possible anti-tümör and apoptotic effects of exogen klotho in human colorectal adenocarcinoma cells (HT-29) and healthy colon cells (CCD 841 CoN). The WST-8 test was used to determine the half-maximum inhibitory concentration (IC50) of the klotho protein. AO-PI fluorescent staining techniques and Annexin V-PI flow cytometry was utilized to observe and detect the apoptosis of cancer cells induced by klotho. Our results demonstrated that klotho had a cytotoxic effect against colorectal adenocarcinoma cells in a dose-dependent manner. Our Annexin V-PI flow cytometric and AO-PI fluorescent analyses showed that klotho induced quantitative and morphological changes that indicate apoptotic induction in the human colorectal adenocarcinoma. This study results proved for the first time that klotho may be an effective potential therapeutic agent that may be used in adjuvant therapy in human colorectal adenocarcinoma it does not affect selectively healthy colon cells and but leading cancer cells to apoptosis.
Klotho 是一种抗衰老、抗炎和抗氧化蛋白，已被证明在几种人类癌症的肿瘤发生、增殖、存活、自噬和对肿瘤抑制作用的抵抗中具有重要作用。在这项研究中，我们旨在研究外源性 Klotho 对人结肠直肠腺癌细胞（HT-29）和健康结肠细胞（CCD 841 CoN）的可能抗肿瘤和促凋亡作用。使用 WST-8 试验来确定 Klotho 蛋白的半最大抑制浓度（IC50）。使用 AO-PI 荧光染色技术和 Annexin V-PI 流式细胞术观察和检测 Klotho 诱导的癌细胞凋亡。我们的结果表明，Klotho 对结肠直肠腺癌细胞具有剂量依赖性的细胞毒性作用。我们的 Annexin V-PI 流式细胞术和 AO-PI 荧光分析表明，Klotho 诱导了定量和形态变化，表明人结肠直肠腺癌细胞的凋亡诱导。这项研究结果首次证明，Klotho 可能是一种有效的潜在治疗剂，可用于人类结肠直肠腺癌的辅助治疗，它不会选择性地影响健康的结肠细胞，而是导致癌细胞凋亡。
