探讨遗传相关中间表型与慢性前列腺炎发病风险之间的因果关系：孟德尔随机化研究。
Investigating Causal Relations between Genetic-Related Intermediate Endophenotype and Risk of Chronic Prostatitis: Mendelian Randomization Study.
机构信息
Department of Biochemistry and Molecular Biology, School of Pre-Clinical Medicine, Guangxi Medical University, Nanning, 53002 Guangxi, China.
Department of Intensive Care Unit, The People's Hospital of Guangxi Zhuang Autonomous Region & Research Center of Intensive Care Unit, Nanning, 530021 Guangxi, China.
出版信息
Oxid Med Cell Longev. 2022 Aug 28;2022:4560609. doi: 10.1155/2022/4560609. eCollection 2022.
OBJECTIVE
Prostatitis is a common disease of the male genitourinary system, which seriously disturbs the physical and mental health of male patients. It is related to many factors such as living habits, age, and race, but the etiology has not been fully elucidated. This study investigated whether there is a causal relationship between clinical biochemical indicators (i.e., intermediate phenotype) and prostatitis through Mendelian randomization. The subjects of the study were prostatitis patients and related SNPs in the Guangxi Fangchenggang health examination cohort.
METHODS
According to the requirements of Mendelian randomization (MR), the single nucleotide polymorphisms (SNPs) related to prostatitis patients and 29 common SNPs related to clinical biochemical indicators were analyzed by linkage disequilibrium, and the calculated SNPs were selected. Finally, the related SNPs were analyzed by Mendelian randomization method.
RESULTS
15 biochemical indicators such as complement C4, FOL, CRP, HCY, and estradiol have shared chronic prostatitis SNP sites, and five qualified SNPs were finally screened for complement C4. Finally, complement C4 was obtained by Mendelian randomization method ( = 0.039), which was statistically significant. The other 28 clinical endophenotypes were all negative.
CONCLUSION
The results show that there was a causal relationship between complement C4 and prostatitis, and the more consistent SNP is rs2075799.
目的
前列腺炎是男性泌尿生殖系统的常见病，严重干扰男性患者的身心健康。它与生活习惯、年龄和种族等诸多因素有关，但病因尚未完全阐明。本研究通过孟德尔随机化（Mendelian randomization）研究临床生化指标（即中间表型）与前列腺炎之间是否存在因果关系。研究对象为前列腺炎患者及广西防城港市健康体检队列中的相关 SNP。
方法
根据孟德尔随机化（MR）的要求，通过连锁不平衡分析与前列腺炎患者相关的单核苷酸多态性（SNPs）和 29 个与临床生化指标相关的常见 SNPs，并计算出相关 SNP。最后，采用孟德尔随机化方法对相关 SNP 进行分析。
结果
15 种生化指标，如补体 C4、FOL、CRP、HCY 和雌二醇，均具有慢性前列腺炎 SNP 共享位点，最终筛选出补体 C4 的 5 个合格 SNP。最后，通过 Mendelian randomization 方法获得补体 C4（ = 0.039），差异有统计学意义。其余 28 个临床内表型均为阴性。
结论
结果表明补体 C4 与前列腺炎之间存在因果关系，且一致性更强的 SNP 是 rs2075799。
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