Zhao Yanteng, Jin Lu, Liu Xin, Liu Xue, Dong Shuling, Chen Yun, Li Xianyu, Lv Xianping, He Meng
Department of Transfusion, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.
Department of Biomedical Engineering and Hubei Province Key Laboratory of Allergy and Immune Related Diseases, School of Basic Medical Sciences, Wuhan University, Wuhan, China.
Front Chem. 2022 Aug 22;10:984652. doi: 10.3389/fchem.2022.984652. eCollection 2022.
High strength polyvinyl alcohol (PVA)/soy protein isolate (SPI) composite hydrogels (EPSG) were constructed by the introduction of PVA into SPI through the crosslinking with epichlorohydrin (ECH) and a freezing-thawing process. The EPSG hydrogels were characterized by scanning electron microscopy, FTIR, X-ray diffraction and compressive test. The results revealed that chemical crosslinking interactions occurred for SPI and PVA during the fabrication process. The composite hydrogels exhibited a homogenous porous structure, indicating certain miscibility between PVA and SPI. The introduction of PVA increased the compressive strength of SPI hydrogels greatly, which could reach as high as 5.38 MPa with the water content ratio of 89.5%. Moreover, the water uptake ratio of completely dried SPI hydrogel (namely xerogel) decreased gradually from 327.4% to 148.1% with the incorporation of PVA, showing a better potential as implants. The cytocompatibility and hemocompatibility of the EPSG hydrogels were evaluated by a series of experiments. The results showed that the EPSG hydrogels had no cytotoxicity (cell viability values were above 86.7%), good biocompatibility and hemocompatibility, showing potential applications as a direct blood contact material in the field of tissue engineering.
通过用环氧氯丙烷（ECH）交联并经过冻融过程将聚乙烯醇（PVA）引入大豆分离蛋白（SPI）中，构建了高强度聚乙烯醇（PVA）/大豆分离蛋白（SPI）复合水凝胶（EPSG）。通过扫描电子显微镜、傅里叶变换红外光谱、X射线衍射和压缩试验对EPSG水凝胶进行了表征。结果表明，在制备过程中SPI和PVA之间发生了化学交联相互作用。复合水凝胶呈现出均匀的多孔结构，表明PVA和SPI之间具有一定的混溶性。PVA的引入大大提高了SPI水凝胶的抗压强度，在含水量为89.5%时，抗压强度可高达5.38MPa。此外，随着PVA的加入，完全干燥的SPI水凝胶（即干凝胶）的吸水率从327.4%逐渐降低到148.1%，显示出作为植入物的更好潜力。通过一系列实验评估了EPSG水凝胶的细胞相容性和血液相容性。结果表明，EPSG水凝胶无细胞毒性（细胞活力值高于86.7%），具有良好的生物相容性和血液相容性，在组织工程领域作为直接血液接触材料显示出潜在的应用前景。
