College of Chemistry and Molecular Sciences, Wuhan University, 430072, Wuhan, China.
Department of Chemistry and Molecular Biology, University of Gothenburg, 41296, Gothenburg, Sweden.
Angew Chem Int Ed Engl. 2022 Oct 24;61(43):e202210224. doi: 10.1002/anie.202210224. Epub 2022 Sep 29.
Interfacing biological tissues with electronic sensors offers the exciting opportunity to accurately investigate multiple biological processes. Accurate signal collection and application are the foundation of these measurements, but a long-term issue is the signal distortion resulting from the interface gap. The height of the gap is the key characteristic needed to evaluate or model the distortion, but it is difficult to measure. By integrating a pair of nanopores at the electronic sensor plane and measuring the ion conductance between them, we developed a versatile and straightforward strategy to realize the direct cooperative evaluation of the gap height during exocytotic release from adrenal gland tissues. The signaling distortion of this gap has been theoretically evaluated and shows almost no influence on the amperometric recording of exocytosis in a classic "semi-artificial synapse" configuration. This strategy should benefit research concerning various bio/chemical/machine interfaces.
将生物组织与电子传感器相连接，为准确研究多种生物过程提供了令人兴奋的机会。准确的信号采集和应用是这些测量的基础，但一个长期存在的问题是接口间隙导致的信号失真。间隙的高度是评估或建模失真所需的关键特征，但很难测量。通过在电子传感器平面上集成一对纳米孔，并测量它们之间的离子电导率，我们开发了一种通用且直接的策略，以实现对肾上腺组织胞吐释放过程中间隙高度的直接协同评估。该间隙的信号失真已在理论上进行了评估，并且在经典的“半人工突触”配置中对胞吐的安培记录几乎没有影响。该策略应有利于各种生物/化学/机械界面的研究。
