Department of Molecular Biology and Genetics, Faculty of Science, Ataturk University, 25240 Erzurum, Turkey.
Department of Food Science and Technology, Faculty of Veterinary Science, Ataturk University, 25240 Erzurum, Turkey.
An Acad Bras Cienc. 2022 Sep 5;94(suppl 3):e20220151. doi: 10.1590/0001-3765202220220151. eCollection 2022.
Millions of tons of agricultural waste are produced globally every year. A practical solution to this global problem is to convert this waste into value-added products. In this study, endoglucanase enzyme production was carried out by using waste melon peels as a carbon source. To use this important resource, its stubborn structure must be broken down. Rumen bacteria are regarded as unique for this job. Therefore, firstly endoglucanase producing rumen bacteria was isolated and the bacteria with the best activity (OB24) were identified by molecular methods (16S rRNA gene squencing). As a result of the sequence analysis, it was determined that isolate belonged to Exiguobacterium mexicanum. Then, by optimizing the culture conditions, the enzyme production potential was increased. The optimal conditions were determined as 50 g/L MPP, 2g/L yeast extract, 60 h incubation time, pH: 6.0, and 40°C temperature. Under optimized conditions the enzyme activity increased approximately 3.8-fold.
每年全球都会产生数百万吨农业废弃物。解决这个全球性问题的一个实际方法是将这些废物转化为高附加值的产品。在这项研究中，使用废弃的瓜皮作为碳源来生产内切葡聚糖酶。为了利用这一重要资源，必须先将其顽固的结构打破。瘤胃细菌被认为是最适合这项工作的细菌。因此，首先从瘤胃细菌中分离出能够产生内切葡聚糖酶的细菌，然后通过分子方法（16S rRNA 基因测序）对活性最好的细菌（OB24）进行鉴定。通过序列分析，确定该分离物属于极端微小杆菌。然后，通过优化培养条件来提高酶的生产潜力。最佳条件确定为：MPP 50g/L、酵母提取物 2g/L、培养时间 60h、pH：6.0 和温度 40°C。在优化条件下，酶活性提高了约 3.8 倍。
