Hecker Yanina P, Masson Fernando Mogaburu, Armendano Joaquín I, Cora Juan, Olivares Carlos Flores, Gual Ignacio, Pardini Lais, Moore Dadín P, Moré Gastón, Cantón Germán J
Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Rosario, Argentina.
Instituto Nacional de Tecnología Agropecuaria (INTA), Buenos Aires, Argentina.
Acta Parasitol. 2018 Jun 26;63(2):416-421. doi: 10.1515/ap-2018-0048.
The aim of this study was to describe the frequency of ovine specific antibodies to Toxoplasma gondii, Neospora caninum and Sarcocystis spp. and to estimate different transmission routes of these infections. One hundred and thirty Texel sheep and their 117 Texel lambs were included in the study. Serum samples were tested for antibodies to T. gondii, N. caninum and Sarcocystis spp. using IFAT. Toxoplasma gondii seroprevalence was 10.00% in sheep (IC95%: 4.80-15.20%), being higher in adult sheep (≥12 year) than in younger sheep (OR 1.30; 95% CI, 1.10-1.50). N. caninum and Sarcocystis spp. seroprevalences were 1.54% (IC95%: 0.00-5.70) and 72.09% (IC95%: 67.70-82.70), respectively, with no association between age and seropositivity in sheep (P>0.05). T. gondii seroprevalence in lambs was 4.27% (IC95%: 0.61-7.94). No association between T. gondii serological status in sheep and their lambs was detected (P = 0.07). Two T. gondii and Sarcocystis spp. seropositive lambs were euthanized and T. gondii and Sarcocystis spp. DNA was detected by PCR in their tissues. In conclusion, the increase of T. gondii seropositivity in relationship with sheep age and the lack of association between sheep-lamb serological status, suggest that horizontal infection is the main transmission route in this flock as reported before. Due to the low number of N. caninum-seropositive ewes no assumptions can be done about the impact of this parasite in this flock. According with previous reports, the main transmission route for Sarcocystis spp. in this species in the present study was horizontal.
本研究的目的是描述绵羊针对刚地弓形虫、犬新孢子虫和肉孢子虫属的特异性抗体频率，并估计这些感染的不同传播途径。130只特克塞尔绵羊及其117只特克塞尔羔羊被纳入本研究。使用间接荧光抗体试验（IFAT）检测血清样本中针对刚地弓形虫、犬新孢子虫和肉孢子虫属的抗体。绵羊的刚地弓形虫血清阳性率为10.00%（95%置信区间：4.80 - 15.20%），成年绵羊（≥12岁）的阳性率高于幼年绵羊（比值比1.30；95%置信区间，1.10 - 1.50）。犬新孢子虫和肉孢子虫属的血清阳性率分别为1.54%（95%置信区间：0.00 - 5.70）和72.09%（95%置信区间：67.70 - 82.70），绵羊的年龄与血清阳性之间无关联（P>0.05）。羔羊的刚地弓形虫血清阳性率为4.27%（95%置信区间：0.61 - 7.94）。未检测到绵羊及其羔羊的刚地弓形虫血清学状态之间存在关联（P = 0.07）。对两只刚地弓形虫和肉孢子虫属血清阳性的羔羊实施安乐死，并通过聚合酶链反应（PCR）在其组织中检测到刚地弓形虫和肉孢子虫属的DNA。总之，刚地弓形虫血清阳性率随绵羊年龄增加，且绵羊与羔羊血清学状态缺乏关联，这表明如之前报道的那样，水平感染是该羊群中的主要传播途径。由于犬新孢子虫血清阳性母羊数量较少，无法对该寄生虫在该羊群中的影响做出假设。根据之前的报道，本研究中该物种肉孢子虫属的主要传播途径是水平传播。
