Nunez Carla Evelyn Coimbra, Oliveira Joaquim Barreto, Barros-Mazon Silvia de, Zago Vanessa H S, Kaplan Denise Beheregaray, Nakamura Ruy T, Gidlund Magnus Ake, Gomes Erica I L, Cazita Patricia Miralda, Nakandakare Edna, Carmo Helison R, Sposito Andrei C, Faria Eliana Cotta de
Universidade Estadual de Campinas (UNICAMP) - Departamento de Patologia , Campinas , SP - Brasil.
Universidade Estadual de Campinas (UNICAMP) - Laboratório de Aterosclerose e Biologia Vascular (Atherolab), Campinas , SP - Brasil.
Arq Bras Cardiol. 2022 Sep 2;119(5):714-21. doi: 10.36660/abc.20210796.
In the atherosclerotic plaque microenvironment, oxidized phospholipids expressed in the oxidized low-density lipoprotein (oxLDL) surface bind to scavenger receptors of macrophages eliciting foam cell formation and plaque progression. Auto-antibodies against oxLDL (oxLDL-Ab) interact with oxidative epitopes leading to the formation of immune complexes that are unable to interact with macrophage receptors, thus abrogating atherogenesis. Release of oxLDL-Ab by B cells involves interleukin 5 and Th2 response, which in turn are potentiated by HDL. Thereby, we hypothesized that individuals with higher levels of HDL-C may plausibly display elevated titers of oxLDL-Ab.
To evaluate the relationship between HDL-C and oxLDL-Ab levels.
Asymptomatic individuals (n = 193) were grouped according to their HDL-C concentration to one of three categories: low (< 68 mg/dL), intermediate (68 to 80 mg/dL) or high (> 80 mg/dL). P values < 0.05 were considered statistically significant.
Our analysis included 193 individuals (mean age: 47 years; male: 26.3%). Compared to individuals in the lowest HDL-C tertile, those in the highest tertile were older (36 versus 53 years; p = 0.001) and less frequently male (42.6% versus 20.9%; p = 0.001). Mean values of oxLDL-Ab increased as the HDL-C group escalated (0.31, 0.33 and 0.43 units, respectively; p = 0.001 for trend). Simple linear regression found a significant, positive relationship between the independent variable, HDL-C, and the dependent variable, oxLDL-Ab (R = 0.293; p = 0.009). This relation remained significant (R = 0.30; p = 0.044), after adjustment by covariates. Apolipoprotein AI levels were also related to oxLDL-Ab in both simple and adjusted linear regression models.
HDL-C and oxLDL-Ab are independently related.
在动脉粥样硬化斑块微环境中，氧化型低密度脂蛋白（oxLDL）表面表达的氧化磷脂与巨噬细胞的清道夫受体结合，引发泡沫细胞形成和斑块进展。抗oxLDL自身抗体（oxLDL-Ab）与氧化表位相互作用，导致免疫复合物形成，而该免疫复合物无法与巨噬细胞受体相互作用，从而消除动脉粥样硬化的发生。B细胞释放oxLDL-Ab涉及白细胞介素5和Th2反应，而高密度脂蛋白（HDL）又会增强这些反应。因此，我们推测HDL-C水平较高的个体可能会出现oxLDL-Ab滴度升高。
评估HDL-C与oxLDL-Ab水平之间的关系。
将193名无症状个体根据其HDL-C浓度分为三类之一：低（<68mg/dL）、中（68至80mg/dL）或高（>80mg/dL）。P值<0.05被认为具有统计学意义。
我们的分析纳入了193名个体（平均年龄：47岁；男性：26.3%）。与HDL-C最低三分位数的个体相比，最高三分位数的个体年龄更大（36岁对53岁；p=0.001），男性比例更低（42.6%对20.9%；p=0.001）。随着HDL-C组升高，oxLDL-Ab的平均值增加（分别为0.31、0.33和0.43单位；趋势p=0.001）。简单线性回归发现自变量HDL-C与因变量oxLDL-Ab之间存在显著的正相关关系（R=0.293；p=0.009）。在通过协变量调整后，这种关系仍然显著（R=0.30；p=0.044）。在简单和调整后的线性回归模型中，载脂蛋白AI水平也与oxLDL-Ab相关。
HDL-C与oxLDL-Ab独立相关。
