Perkins P S, Young R W
Brain Res. 1987 Jun;430(2):161-8. doi: 10.1016/0165-3806(87)90150-7.
Histone proteins from purified nuclei of neonatal and adult mouse retinas were analyzed and compared utilizing SDS-polyacrylamide gel electrophoresis. Identical procedures were applied to examine the histones extracted from the brains of the same animals. In the newborn and mature retina and brain, 8 histone fractions have been separated, identified and quantified by scanning densitometry. These are the linker histone (H1), consisting of 3 subfractions (H1a, H1b, H1(0); the semi-histone uH2A (A24); and the 4 nucleosome core histones (H2A, H2B, H3, H4). Developmental differences are exhibited by the linker histone in both brain and retinal cells. The greatest differences between the histone patterns of retina and brain are also in the H1 group. Because the linker histone is subject to the greatest variability. H1 was selectively extracted with 5% perchloric acid from both neonatal and adult brain. This procedure established that the observed differences are a developmental phenomenon and are not due to interactions of the linker histones with other nuclear proteins. The ratio of the non-histone chromosomal proteins to total histone was found to be significantly greater in both adult and neonatal brain compared to retina at either age.
利用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳对新生小鼠和成年小鼠视网膜纯化细胞核中的组蛋白进行了分析和比较。采用相同的程序检测从相同动物大脑中提取的组蛋白。在新生和成熟的视网膜及大脑中，已通过扫描密度测定法分离、鉴定并定量了8个组蛋白组分。它们分别是连接组蛋白（H1），由3个亚组分组成（H1a、H1b、H1(0)）；半组蛋白uH2A（A24）；以及4种核小体核心组蛋白（H2A、H2B、H3、H4）。连接组蛋白在大脑和视网膜细胞中均表现出发育差异。视网膜和大脑组蛋白模式之间的最大差异也存在于H1组中。因为连接组蛋白的变异性最大。用5%的高氯酸从新生和成年大脑中选择性提取H1。该程序表明观察到的差异是一种发育现象，并非由于连接组蛋白与其他核蛋白的相互作用所致。与任一年龄段的视网膜相比，成年和新生大脑中，非组蛋白染色体蛋白与总组蛋白的比率均显著更高。
