代谢组学揭示了低压处理对振动损伤‘黄冠’梨能量代谢的影响。

Metabolomics reveals the effect of hypobaric treatment on energy metabolism in vibration-injured 'Huangguan' pears.

机构信息

Experimental Teaching Center, Shenyang Normal University, Shenyang, Liaoning 110034, People's Republic of China.

出版信息

Food Chem. 2023 Jan 30;400:134057. doi: 10.1016/j.foodchem.2022.134057. Epub 2022 Sep 5.

DOI:10.1016/j.foodchem.2022.134057

Abstract

Mechanical damage caused by vibration during transportation can destroy organization structure and reduce the fruit quality. The objective was to reveal the mechanism of hypobaric treatment on energy metabolism in vibration-injured 'Huangguan' pears based on metabolomics. Results showed that hypobaric treatment delayed the decline of adenosine triphosphate (ATP) content, energy charge (EC), H-ATPase and Ca-ATPase activities comparing to untreated samples. Metabolomics data indicated there were 83 significant differential metabolites between untreated samples and hypobaric treated ones. KEGG analysis results showed significant differential metabolites were associated with 14 pathways. Key metabolites and pathways analysis revealed these up-regulated amino acids were related to amino acid metabolism, biosynthesis of secondary metabolites and membrane transport. These pathways were activated observably by hypobaric treatment. The results indicated hypobaric treatment slowed energy consumption in vibration-injured samples, which was in relation to the accumulation of amino acids. The findings provide a feasible preservation technology for vibration-injured fruit.

摘要

运输过程中振动造成的机械损伤会破坏组织结构，降低果实品质。本研究旨在基于代谢组学揭示减压处理对振动损伤‘黄冠’梨能量代谢的作用机制。结果表明，与未处理的样品相比，减压处理延迟了三磷酸腺苷（ATP）含量、能量荷（EC）、H-ATP 酶和 Ca-ATP 酶活性的下降。代谢组学数据表明，未处理的样品和减压处理的样品之间有 83 个显著差异的代谢物。KEGG 分析结果表明，显著差异的代谢物与 14 条途径有关。关键代谢物和途径分析表明，这些上调的氨基酸与氨基酸代谢、次生代谢物的生物合成和膜转运有关。这些途径在减压处理下被明显激活。结果表明，减压处理减缓了振动损伤样品的能量消耗，这与氨基酸的积累有关。研究结果为振动损伤果实提供了一种可行的保鲜技术。