Lan Qian, Xiao Xin, Bi Xueting, Gu Yangcong, Ai Yilong
Department of Periodontics, Foshan Stomatology Hospital and School of Medicine, Foshan University, Foshan, Guangdong Province, China.
Department of Maxillofacial Surgery, Foshan Stomatology Hospital and School of Medicine, Foshan University, Foshan, Guangdong Province, China.
Oral Dis. 2024 Mar;30(2):710-718. doi: 10.1111/odi.14375. Epub 2022 Sep 27.
Periodontitis is characterized by alveolar bone injury and absorption, with high incidence and poor treatment effect. Proliferation, migration, differentiation and apoptosis of osteoblasts are identified as key factors during the regeneration of alveolar bone tissue processes. Periodontal ligament stem cells (PDLSCs) have been proved to be a possible candidate for the treatment of periodontitis due to its multiple advantages, such as increasing the regenerative capacity of bone tissue. However, the effect of exosomes derived from PDLSCs (PDLSC-Exo) on osteoblasts remains to be further studied.
In this work, cell proliferation, migration, osteogenic differentiation, and H O -induced apoptosis were detected after cells were exposed to PDLSC-Exo by CCK-8, scratch wound assay, alizarin red S and alkaline phosphatase staining, real-time PCR, flow cytometry, tunel assay, and so on. Moreover, the activation of PI3K/AKT and MEK/ERK signaling pathways was evaluated by western blotting.
We found that PDLSC-Exo are capable of promoting hFOB1.19 cell proliferation, migration and osteogenic differentiation, inhibiting H O -induced apoptosis, and activating the PI3K/AKT and MEK/ERK signaling pathways.
These results suggest that PDLSC-Exo may be a promising therapeutic for osteoblastic damage.
牙周炎以牙槽骨损伤和吸收为特征，发病率高且治疗效果不佳。成骨细胞的增殖、迁移、分化和凋亡被认为是牙槽骨组织再生过程中的关键因素。牙周膜干细胞（PDLSCs）因其具有多种优势，如增强骨组织再生能力，已被证明是治疗牙周炎的一种可能选择。然而，源自牙周膜干细胞的外泌体（PDLSC-Exo）对成骨细胞的影响仍有待进一步研究。
在本研究中，通过CCK-8、划痕试验、茜素红S和碱性磷酸酶染色、实时PCR、流式细胞术、TUNEL检测等方法，检测细胞暴露于PDLSC-Exo后的细胞增殖、迁移、成骨分化及H₂O₂诱导的凋亡情况。此外，通过蛋白质免疫印迹法评估PI3K/AKT和MEK/ERK信号通路的激活情况。
我们发现PDLSC-Exo能够促进hFOB1.19细胞增殖、迁移和成骨分化，抑制H₂O₂诱导的凋亡，并激活PI3K/AKT和MEK/ERK信号通路。
这些结果表明，PDLSC-Exo可能是一种有前景的治疗成骨细胞损伤的方法。
