School of Bacical Medical Sciences, Department of Pharmacology, Shanxi Medical University, Taiyuan 030000, China.
Department of Orthopedics, The First Hospital of Shanxi Medical University, Taiyuan 030000, China.
Biomed Pharmacother. 2022 Sep;153:113530. doi: 10.1016/j.biopha.2022.113530. Epub 2022 Aug 11.
To verify the role of myricetin in alleviating the symptoms of type 2 diabetes and regulating the intestinal flora, we established a type 2 diabetes mouse model. After being fed a high-fat and high-sugar diet for six weeks, mice were intraperitoneally injected with streptozotocin (80 mg/kg body weight [BW]) 2-3 times. Type 2 diabetes mice were randomly divided into type 2 diabetes control (T2DM) and myricetin intervention groups. Water and food intake, fasting blood glucose (FBG), and BW were monitored weekly. After six weeks of myricetin administration, superoxide dismutase (SOD) levels and blood lipid content were measured. Furthermore, 16S rRNA sequencing was used to detect the gut microbiota composition. FBG and blood lipid levels of T2DM mice were significantly reduced upon myricetin treatment, while SOD levels were increased. Myricetin improved polydipsia, polyphagia, polyuria, and weight loss in T2DM mice. In addition, the signature type 2 diabetes microflora was established by analyzing the microflora structure of healthy mice, type 2 diabetes mice, and mice treated with myricetin. Results showed that type 2 diabetes disrupted the mice intestinal flora, and myricetin intervention normalized the intestinal flora. In conclusion, our results indicate that myricetin alleviates type 2 diabetes in mice and regulates the intestinal microflora.
为了验证杨梅素缓解 2 型糖尿病症状和调节肠道菌群的作用，我们建立了 2 型糖尿病小鼠模型。经过 6 周的高脂高糖饮食喂养后，小鼠腹腔内注射链脲佐菌素（80mg/kg 体重[BW]）2-3 次。将 2 型糖尿病小鼠随机分为 2 型糖尿病对照组（T2DM）和杨梅素干预组。每周监测水和食物摄入量、空腹血糖（FBG）和 BW。杨梅素给药 6 周后，测量超氧化物歧化酶（SOD）水平和血脂含量。此外，使用 16S rRNA 测序检测肠道微生物群落组成。杨梅素治疗可显著降低 T2DM 小鼠的 FBG 和血脂水平，同时 SOD 水平升高。杨梅素改善了 T2DM 小鼠的多饮、多食、多尿和体重减轻症状。此外，通过分析健康小鼠、2 型糖尿病小鼠和用杨梅素治疗的小鼠的微生物群落结构，确定了 2 型糖尿病特征性微生物群。结果表明，2 型糖尿病破坏了小鼠的肠道菌群，杨梅素干预使肠道菌群正常化。总之，我们的结果表明杨梅素缓解了小鼠的 2 型糖尿病并调节了肠道微生物群。
