Zhang Kai, Phillips Melissa
Food and Drug Administration, Center for Food Safety and Applied Nutrition, 5001 Campus Drive, College Park, MD 20747, USA.
National Institute of Standards and Technology, 100 Bureau Drive, Gaithersburg, MD 20899, USA.
Foods. 2022 Aug 23;11(17):2544. doi: 10.3390/foods11172544.
The analysis of mycotoxins in food and feed using liquid chromatography coupled with mass spectrometry is considered advantageous because the hyphenated technology enables simultaneous determination of multiple mycotoxins. Multi-mycotoxin analysis requires special consideration of quality control parameters to ensure proper evaluation of data quality for all target mycotoxins in method development and routine sample analysis. Mycotoxin matrix reference materials, especially certified reference materials, are stable and homogeneous matrices with certified traceability, concentrations, and uncertainty for mycotoxin(s) of interest. The use of these reference materials for single mycotoxin analysis has been a well-accepted practice and should be extended to multi-mycotoxin analysis. This opinion piece discusses the following essential metrological and operational components to improve data quality: (1) purposes of multi-mycotoxin reference materials; (2) comparison of reference materials, certified reference materials, and in-house quality control materials; (3) advantages of using reference materials for multi-mycotoxin analysis; (4) current trends and challenges of multi-mycotoxin reference materials. Potential applications of reference materials discussed here can improve routine mycotoxin determination and will lead to better accuracy and consistency of results. Quality control processes that incorporate reference materials in the field of mycotoxin analysis ensure successful development and implementation of liquid chromatography mass spectrometry-based multi-mycotoxin methods.
使用液相色谱-质谱联用技术分析食品和饲料中的霉菌毒素被认为具有优势，因为这种联用技术能够同时测定多种霉菌毒素。多霉菌毒素分析需要特别考虑质量控制参数，以确保在方法开发和常规样品分析中对所有目标霉菌毒素的数据质量进行恰当评估。霉菌毒素基质参考物质，尤其是有证参考物质，是具有稳定且均匀的基质，其对感兴趣的霉菌毒素具有经认证的可溯源性、浓度和不确定度。将这些参考物质用于单一霉菌毒素分析已是一种广泛接受的做法，并且应扩展到多霉菌毒素分析。这篇观点文章讨论了以下提高数据质量的重要计量和操作要素：（1）多霉菌毒素参考物质的用途；（2）参考物质、有证参考物质和内部质量控制物质的比较；（3）使用参考物质进行多霉菌毒素分析的优势；（4）多霉菌毒素参考物质的当前趋势和挑战。本文讨论的参考物质的潜在应用可改善常规霉菌毒素测定，并将带来更高的结果准确性和一致性。在霉菌毒素分析领域纳入参考物质的质量控制流程可确保基于液相色谱-质谱联用的多霉菌毒素方法的成功开发和实施。
