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粉末对低脂猪肉肉糜的理化和质地特性、水分分布及蛋白质构象的影响

Effect of Powder on Physicochemical and Textural Properties, Water Distribution and Protein Conformation of Lower-Fat Pork Meat Batter.

作者信息

Zhao Yingying, Wang Yanqiu, Li Ke, Mazurenko Igor

机构信息

College of Food and Bioengineering, Zhengzhou University of Light Industry, Zhengzhou 450001, China.

Department of Food Technology, Sumy National Agrarian University, 40021 Sumy, Ukraine.

出版信息

Foods. 2022 Aug 29;11(17):2623. doi: 10.3390/foods11172623.

Abstract

The effects of the addition of different amounts (0%, 1%, 2%, 3% and 4%) of Oudemansiella raphanipies powder (ORP) to lower-fat pork batter on its physicochemical, textural and rheological properties, water distribution and protein conformation were evaluated. The results showed that the addition of ORP from 0% to 4% significantly decreased the pH and L* value of pork batter (p < 0.05); however, it also increased the a* value and enhanced the cooking yield of pork batter from 77% to 92%. Pork batter with 1−2% ORP added had an improved texture profile and a higher storage modulus (G’), but the addition of 3−4% ORP resulted in an inferior texture of pork batter and G’. LF-NMR showed that the addition of ORP significantly increased the peak area ratio of immobile water and reduced the peak area ratio of free water (p < 0.05). ORP significantly affected protein secondary structure of pork batter. The α-helix content of pork batter with 1−2% ORP decreased and β-sheet content increased. Overall, the addition level of 1−2% ORP effectively improved the texture and water holding capacity of lower-fat emulsified sausage and provides a new reference for developing nutritional meat products.

摘要

研究了在低脂猪肉肠衣中添加不同量(0%、1%、2%、3%和4%)的长根奥德蘑粉(ORP)对其理化性质、质地、流变学性质、水分分布和蛋白质构象的影响。结果表明,添加0%至4%的ORP显著降低了猪肉肠衣的pH值和L值(p<0.05);然而,它也增加了a值,并将猪肉肠衣的蒸煮得率从77%提高到92%。添加1-2%ORP的猪肉肠衣具有改善的质地剖面和更高的储能模量(G’),但添加3-4%ORP会导致猪肉肠衣的质地和G’变差。低场核磁共振(LF-NMR)表明,添加ORP显著增加了结合水的峰面积比,降低了自由水的峰面积比(p<0.05)。ORP显著影响猪肉肠衣的蛋白质二级结构。添加1-2%ORP的猪肉肠衣α-螺旋含量降低,β-折叠含量增加。总体而言,1-2%的ORP添加量有效改善了低脂乳化香肠的质地和持水能力,为开发营养肉类产品提供了新的参考。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fff6/9455643/fea823798a25/foods-11-02623-g001.jpg

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