College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China.
Int J Mol Sci. 2022 Aug 23;23(17):9545. doi: 10.3390/ijms23179545.
Proliferation, differentiation, and apoptosis are three essential stages in cell development, and miRNAs can achieve extensive regulation of cellular developmental processes by repressing the expression of target genes. According to our previous RNA-seq results, miRNA-10a-5p was differentially expressed at different periods in chicken myoblasts, revealing a possible association with muscle development. In this study, we concluded that miRNA-10a-5p inhibited chicken myoblasts' proliferation and differentiation and promoted chicken myoblasts' apoptosis by directly targeting BCL6, a critical transcription factor involved in muscle development and regeneration. Overexpression of BCL6 significantly facilitated myoblasts' proliferation and differentiation and suppressed myoblasts' apoptosis. On the contrary, knockdown of BCL6 significantly repressed myoblasts' proliferation and differentiation and induced myoblasts' apoptosis. The results above suggest that miRNA-10a-5p plays a potential role in skeletal muscle growth, development and autophagy by targeting the BCL6 gene. We first revealed the functions of miRNA-10a-5p and BCL6 in the proliferation, differentiation, and apoptosis of chicken myoblasts.
增殖、分化和凋亡是细胞发育的三个重要阶段，miRNA 可以通过抑制靶基因的表达来实现对细胞发育过程的广泛调控。根据我们之前的 RNA-seq 结果，miRNA-10a-5p 在鸡成肌细胞的不同时期表达差异，表明其可能与肌肉发育有关。在本研究中，我们得出结论，miRNA-10a-5p 通过直接靶向参与肌肉发育和再生的关键转录因子 BCL6，抑制鸡成肌细胞的增殖和分化，促进鸡成肌细胞的凋亡。BCL6 的过表达显著促进了成肌细胞的增殖和分化，并抑制了成肌细胞的凋亡。相反，BCL6 的敲低显著抑制了成肌细胞的增殖和分化，并诱导了成肌细胞的凋亡。上述结果表明，miRNA-10a-5p 通过靶向 BCL6 基因在骨骼肌的生长、发育和自噬中发挥潜在作用。我们首次揭示了 miRNA-10a-5p 和 BCL6 在鸡成肌细胞增殖、分化和凋亡中的功能。
