Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (Northeast Region), Ministry of Agriculture and Rural Affairs/National-Local Joint Engineering Research Center for Development and Utilization of Small Fruits in Cold Regions/College of Horticulture & Landscape Architecture, Northeast Agricultural University, Harbin 150030, China.
Horticulture Branch of Heilongjiang Academy of Agricultural Sciences, Harbin 150040, China.
Int J Mol Sci. 2022 Aug 29;23(17):9827. doi: 10.3390/ijms23179827.
CBF transcription factors (TFs) are key regulators of plant stress tolerance and play an integral role in plant tolerance to adverse growth environments. However, in the current research situation, there are few reports on the response of the CBF gene to Begonia stress. Therefore, this experiment investigated a novel CBF TF gene, named , which was isolated from seedlings. According to the subcellular localization results, the MbCBF2 protein was located in the nucleus. In addition, the expression level of was higher in new leaves and roots under low-temperature and high-salt induction. After the introduction of into , the adaptability of transgenic to cold and high-salt environments was significantly enhanced. In addition, the high expression of can also change many physiological indicators in transgenic , such as increased chlorophyll and proline content, superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) activity, and reduced malondialdehyde (MDA) content. Therefore, it can be seen from the above results that can positively regulate the response of to low-temperature and osmotic stress. In addition, can also regulate the expression of its downstream genes in transgenic lines. It can not only positively regulate the expression of the downstream key genes , , and , related to cold stress at low temperatures, but can also positively regulate the expression of the downstream key genes , , , and , related to salt stress. That is, the overexpression of the gene further improved the adaptability and tolerance of transgenic plants to low-temperature and high-salt environments.
CBF 转录因子（TFs）是植物应激耐受的关键调节因子，在植物耐受不利生长环境方面发挥着重要作用。然而，在当前的研究情况下，关于 CBF 基因对应对秋海棠胁迫的反应的报道很少。因此，本实验研究了一种新型 CBF TF 基因，命名为 ，它从 幼苗中分离得到。根据亚细胞定位结果，MbCBF2 蛋白位于细胞核内。此外，在低温和高盐诱导下， 基因在新叶和根中的表达水平较高。将 导入 后，转基因 对低温和高盐环境的适应性显著增强。此外， 基因的高表达还可以改变转基因 中的许多生理指标，如增加叶绿素和脯氨酸含量、超氧化物歧化酶（SOD）、过氧化物酶（POD）和过氧化氢酶（CAT）活性，以及降低丙二醛（MDA）含量。因此，从以上结果可以看出， 可以正向调节 对低温和渗透胁迫的响应。此外， 还可以调节转基因系中其下游基因的表达。它不仅可以正向调节低温下与冷胁迫相关的下游关键基因 、 、 和 的表达，还可以正向调节与盐胁迫相关的下游关键基因 、 、 、 和 的表达。也就是说， 基因的过表达进一步提高了转基因植物对低温和高盐环境的适应能力和耐受能力。
