Laboratory Animal Resource Center in Transborder Medical Research Center, Faculty of Medicine, University of Tsukuba, 1-1-1 Tennodai, Tsukuba 305-8575, Japan.
Department of Pathology, Faculty of Veterinary Medicine, Mansoura University, Mansoura 35516, Egypt.
Int J Mol Sci. 2022 Sep 1;23(17):9945. doi: 10.3390/ijms23179945.
MAFB is a basic leucine zipper (bZIP) transcription factor specifically expressed in macrophages. We have previously identified MAFB as a candidate marker for tumor-associated macrophages (TAMs) in human and mouse models. Here, we analyzed single-cell sequencing data of patients with lung adenocarcinoma obtained from the GEO database (GSE131907). Analyzed data showed that general macrophage marker CD68 and macrophage scavenger receptor 1 (CD204) were expressed in TAM and lung tissue macrophage clusters, while transcription factor MAFB was expressed specifically in TAM clusters. Clinical records of 120 patients with lung adenocarcinoma stage I ( = 57), II ( = 21), and III ( = 42) were retrieved from Tsukuba Human Tissue Biobank Center (THB) in the University of Tsukuba Hospital, Japan. Tumor tissues from these patients were extracted and stained with anti-human MAFB antibody, and then MAFB-positive cells relative to the tissue area (MAFB cells/tissue area) were morphometrically quantified. Our results indicated that higher numbers of MAFB cells significantly correlated to increased local lymph node metastasis (nodal involvement), high recurrence rate, poor pathological stage, increased lymphatic permeation, higher vascular invasion, and pleural infiltration. Moreover, increased amounts of MAFB cells were related to poor overall survival and disease-free survival, especially in smokers. These data indicate that MAFB may be a suitable prognostic biomarker for smoker lung cancer patients.
MAFB 是一种碱性亮氨酸拉链 (bZIP) 转录因子，特异性表达于巨噬细胞。我们之前已将 MAFB 鉴定为人类和小鼠模型中肿瘤相关巨噬细胞 (TAM) 的候选标志物。在此，我们分析了从 GEO 数据库 (GSE131907) 获取的肺腺癌患者的单细胞测序数据。分析数据显示，通用巨噬细胞标志物 CD68 和巨噬细胞清道夫受体 1 (CD204) 在 TAM 和肺组织巨噬细胞簇中表达，而转录因子 MAFB 特异性表达于 TAM 簇中。从日本筑波大学医院筑波人类组织生物库中心 (THB) 检索了 120 例肺腺癌 I 期（n = 57）、II 期（n = 21）和 III 期（n = 42）患者的临床记录。从这些患者的肿瘤组织中提取并用抗人 MAFB 抗体染色，然后对组织面积的 MAFB 阳性细胞（MAFB 细胞/组织面积）进行形态计量学定量。我们的结果表明，较高数量的 MAFB 细胞与局部淋巴结转移（淋巴结受累）增加、高复发率、较差的病理分期、淋巴管渗透增加、血管侵犯增加和胸膜浸润增加显著相关。此外，MAFB 细胞数量的增加与较差的总生存期和无病生存期相关，尤其是在吸烟者中。这些数据表明，MAFB 可能是适合吸烟肺癌患者的预后生物标志物。
