Department of Thoracic Surgery, The First Hospital of China Medical University, Shenyang, China.
Biomed Res Int. 2018 Apr 16;2018:8459193. doi: 10.1155/2018/8459193. eCollection 2018.
Macrophages are the dominant leukocytes in the tumor microenvironment. Accumulating evidence revealed that CD204-positive (CD204+) tumor-associated macrophages (TAMs) are associated with the aggressive behavior of various cancers; however, the clinical, pathological, and prognostic associations of CD204+ TAMs with the subtype of lung adenocarcinoma have not been reported.
Tissue microarray and immunohistochemistry were constructed from clinical stage I lung adenocarcinomas with radical surgical resection. The intratumoral density of CD204+ cells was calculated using image analysis software for analyses. Survival analyses were performed using the Kaplan-Meier method and multivariate Cox proportional hazards regression models.
The intratumoral density of CD204 was correlated with T stage, nodal involvement, lymphovascular invasion, and cancer relapse after the surgery, but not with age, gender, or smoking history. The density of CD204 in non-LPD was significantly higher than that in LPD. The 5-year disease-free survival (DFS) rate of CD204 high-density group was significantly worse than that of CD204 low-density group.
The expression of CD204 in TAMs is associated with the aggressiveness of lung adenocarcinoma. Our results suggest that a specific immune microenvironment may be associated with the biological behavior of lung adenocarcinoma.
巨噬细胞是肿瘤微环境中的主要白细胞。越来越多的证据表明，CD204 阳性（CD204+）肿瘤相关巨噬细胞（TAMs）与各种癌症的侵袭性行为有关；然而，CD204+TAMs 与肺腺癌亚型的临床、病理和预后关联尚未报道。
使用具有根治性手术切除的临床 I 期肺腺癌构建组织微阵列和免疫组织化学。使用图像分析软件计算 CD204+细胞的肿瘤内密度以进行分析。使用 Kaplan-Meier 方法和多变量 Cox 比例风险回归模型进行生存分析。
CD204 的肿瘤内密度与 T 分期、淋巴结受累、血管淋巴管侵犯以及手术后癌症复发相关，但与年龄、性别或吸烟史无关。非 LPD 中的 CD204 密度明显高于 LPD。CD204 高密度组的 5 年无病生存率（DFS）明显差于 CD204 低密度组。
TAMs 中 CD204 的表达与肺腺癌的侵袭性有关。我们的结果表明，特定的免疫微环境可能与肺腺癌的生物学行为有关。
