Department of Cardiothoracic Anaesthesia and Intensive Care, The James Cook University Hospital, Marton Road, Middlesbrough TS4 3BW, UK.
First Faculty of Medicine, Charles University, Katerinska 32, 121 08 Prague, Czech Republic.
Int J Mol Sci. 2022 Sep 1;23(17):9988. doi: 10.3390/ijms23179988.
(1) Background: C1q TNF-related protein 3 (CTRP3) is an adipokine with anti-inflammatory and cardioprotective properties. In our study, we explored changes in serum CTRP3 and its gene expression in epicardial (EAT) and subcutaneous (SAT) adipose tissue in patients with and without coronary artery disease (CAD) and type 2 diabetes mellitus (T2DM) undergoing elective cardiac surgery. (2) Methods: SAT, EAT, and blood samples were collected at the start and end of surgery from 34 patients: (i) 11 without CAD or T2DM, (ii) 14 with CAD and without T2DM, and (iii) 9 with both CAD and T2DM. mRNA levels of were assessed by quantitative reverse transcription PCR. Circulating levels of CTRP3 and other factors were measured using ELISA and Luminex Multiplex commercial kits. (3) Results: Baseline plasma levels of TNF-α and IL6 did not differ among the groups and increased at the end of surgery. Baseline circulating levels of CTRP3 did not differ among the groups and decreased after surgery. In contrast, baseline mRNA levels in EAT were significantly decreased in CAD/T2DM group, while no differences were found for and gene expression. (4) Conclusions: Our data suggest that decreased EAT mRNA levels of could contribute to higher risk of atherosclerosis in patients with CAD and T2DM.
（1）背景：C1q 肿瘤坏死因子相关蛋白 3（CTRP3）是一种具有抗炎和心脏保护特性的脂肪因子。在我们的研究中，我们探讨了接受择期心脏手术的冠心病（CAD）和 2 型糖尿病（T2DM）患者的心外膜（EAT）和皮下（SAT）脂肪组织中血清 CTRP3 及其基因表达的变化。（2）方法：从 34 例患者中采集 SAT、EAT 和血液样本：（i）11 例无 CAD 或 T2DM，（ii）14 例有 CAD 但无 T2DM，（iii）9 例既有 CAD 又有 T2DM。采用定量逆转录 PCR 评估 的 mRNA 水平。使用 ELISA 和 Luminex 多重商业试剂盒测量循环 CTRP3 水平和其他因子。（3）结果：基线时 TNF-α和 IL6 血浆水平在各组之间无差异，并在手术结束时升高。基线时循环 CTRP3 水平在各组之间无差异，并在手术后下降。相比之下，CAD/T2DM 组 EAT 中 的基线 mRNA 水平显著降低，而 和 基因表达则无差异。（4）结论：我们的数据表明，CAD 和 T2DM 患者 EAT 中 的 mRNA 水平降低可能导致动脉粥样硬化风险增加。
