Matloch Z, Kratochvílová H, Cinkajzlová A, Lipš M, Kopecký P, Pořízka M, Haluzíková D, Lindner J, Mráz M, Kloučková J, Lacinová Z, Haluzík M
Dr. Pirek's Clinic, Mlada Boleslav, Czech Republic, Center for Experimental Medicine, Institute for Clinical and Experimental Medicine, Prague, Czech Republic.
Physiol Res. 2018 Dec 18;67(6):881-890. doi: 10.33549/physiolres.933909. Epub 2018 Sep 11.
Omentin is a protein produced by numerous tissues including adipose tissue. Its concentrations are decreased in patients with obesity, type 2 diabetes mellitus (DM) and coronary artery disease (CAD). Experimental studies suggest that omentin may have anti-inflammatory and insulin-sensitizing properties. In the present study, we measured circulating omentin levels and its mRNA expression in epicardial and subcutaneous fat, intercostal and heart muscle before and after elective cardiac surgery in patients with CAD (CAD+, DM-, n=18), combination of CAD and DM (CAD+, DM+, n=9) or with none of these conditions (CAD-, DM-, n=11). The groups did not differ in baseline anthropometric and biochemical characteristics with the exception of higher blood glucose and HBA(1c) in CAD+, DM+ group. Baseline circulating omentin levels tended to be lower in CAD+, DM- and CAD+, DM+ groups as compared to CAD-, DM- group and cardiac surgery increased its concentration only in CAD-, DM- group. The change in serum omentin levels during surgery inversely correlated with epicardial fat thickness. While baseline omentin mRNA expression did not differ among the groups in any of the studied tissues, its increase after surgery was present only in subcutaneous fat in CAD-, DM- and CAD+, DM- groups, but not in CAD+, DM+ group. Intercostal muscle omentin mRNA expression increased after surgery only in CAD-, DM- group. In conclusion, cardiac surgery differentially affects omentin levels and subcutaneous fat and skeletal muscle mRNA expression in patients without coronary artery disease and diabetes as compared to patients with these conditions.
网膜素是一种由包括脂肪组织在内的多种组织产生的蛋白质。肥胖、2型糖尿病（DM）和冠状动脉疾病（CAD）患者的网膜素浓度会降低。实验研究表明，网膜素可能具有抗炎和胰岛素增敏特性。在本研究中，我们测量了CAD患者（CAD+，DM-，n = 18）、CAD和DM合并患者（CAD+，DM+，n = 9）或无这些疾病患者（CAD-，DM-，n = 11）在择期心脏手术前后循环网膜素水平及其在心脏外膜和皮下脂肪、肋间肌和心肌中的mRNA表达。除CAD+，DM+组血糖和糖化血红蛋白（HBA1c）较高外，各组基线人体测量学和生化特征无差异。与CAD-，DM-组相比，CAD+，DM-组和CAD+，DM+组基线循环网膜素水平往往较低，心脏手术仅使CAD-，DM-组的网膜素浓度升高。手术期间血清网膜素水平的变化与心脏外膜脂肪厚度呈负相关。虽然在任何研究组织中，各组间基线网膜素mRNA表达无差异，但手术后其增加仅出现在CAD-，DM-组和CAD+，DM-组的皮下脂肪中，而在CAD+，DM+组中未出现。手术后，仅CAD-，DM-组的肋间肌网膜素mRNA表达增加。总之，与患有这些疾病的患者相比，心脏手术对无冠状动脉疾病和糖尿病患者的网膜素水平以及皮下脂肪和骨骼肌mRNA表达的影响存在差异。
