Laboratory of Stem Cells and Tissue Engineering, Department of Histology and Embryology, Chongqing Medical University, Chongqing 400016, China.
Faculty of Basic Medical Sciences, Chongqing Medical and Pharmaceutical College, Chongqing 401331, China.
Int J Mol Sci. 2022 Sep 2;23(17):10045. doi: 10.3390/ijms231710045.
Ginsenoside Rg1 is an important active substance isolated from the root of ginseng. In previous studies, Rg1 has shown excellent therapeutic effects in antioxidant, anti-inflammatory, and metabolic modulation. However, the therapeutic targets of Rg1 are still unknown. In this study, we investigated the therapeutic effects of Rg1 on oxidative stress-related liver damage. The oxidative stress damage model was achieved by intraperitoneal injection of D-galactose (D-gal) for 42 consecutive days in C57BL/6J mice. Rg1 treatment started on Day 16. Body weight, liver weight, degree of hepatic oxidative stress damage, serum lipid levels, and hepatic lipid and glucose metabolism were measured. Proteomics analysis was used to measure liver protein expression. The differential expression proteins were analyzed with bioinformatics. The results showed that Rg1 treatment attenuated liver damage from oxidative stress, reduced hepatic fat accumulation, promoted hepatic glycogen synthesis, and attenuated peripheral blood low-density lipoprotein (LDL), cholesterol (CHO), and triglycerides (TG) levels. Proteomic analysis suggested that Rg1 may regulate hepatocyte metabolism through ECM-Receptor, the PI3K-AKT pathway. The epidermal growth factor receptor (EGFR) and activator of transcription 1 (STAT1) may be the key protein. In conclusion, this study provides an experimental basis for further clarifying the specific mechanism of Rg1 in the treatment of oxidative stress damage-related liver disease.
人参皂苷 Rg1 是从人参根中分离得到的一种重要活性物质。在以前的研究中，Rg1 在抗氧化、抗炎和代谢调节方面显示出了极好的治疗效果。然而，Rg1 的治疗靶点仍不清楚。在本研究中，我们研究了 Rg1 对氧化应激相关肝损伤的治疗作用。通过连续 42 天腹腔注射 D-半乳糖（D-gal）在 C57BL/6J 小鼠中建立氧化应激损伤模型。Rg1 治疗从第 16 天开始。测量体重、肝重、肝氧化应激损伤程度、血清脂质水平以及肝脂和糖代谢。采用蛋白质组学分析测量肝脏蛋白表达。使用生物信息学分析差异表达蛋白。结果表明，Rg1 治疗可减轻氧化应激引起的肝损伤，减少肝脂肪堆积，促进肝糖原合成，并减轻外周血低密度脂蛋白（LDL）、胆固醇（CHO）和甘油三酯（TG）水平。蛋白质组学分析表明，Rg1 可能通过细胞外基质受体（ECM-Receptor）、PI3K-AKT 通路调节肝细胞代谢。表皮生长因子受体（EGFR）和转录激活因子 1（STAT1）可能是关键蛋白。总之，本研究为进一步阐明 Rg1 治疗氧化应激损伤相关肝病的具体机制提供了实验依据。
