The Key Laboratory for Quality Improvement of Agricultural Products of Zhejiang Province, College of Advanced Agricultural Sciences, Zhejiang A & F University, Hangzhou 311300, China.
Int J Mol Sci. 2022 Sep 5;23(17):10182. doi: 10.3390/ijms231710182.
NAC transcription factors play crucial roles in plant growth, development and stress responses. Previously, we preliminarily identified that the transcription factor gene was significantly up-regulated under salt stress in okra (). Herein, we cloned the nuclear-localized AeNAC83 from okra and identified its possible role in salt stress response and plant growth. The down-regulation of caused by virus-induced gene silencing enhanced plant sensitivity to salt stress and increased the biomass accumulation of okra seedlings. Meanwhile, -overexpression lines improved salt tolerance and exhibited many altered phenotypes, including small rosette, short primary roots, and promoted crown roots and root hairs. RNA-seq showed numerous genes at the transcriptional level that changed significantly in the -overexpression transgenic and the wild with or without NaCl treatment, respectively. The expression of most phenylpropanoid and flavonoid biosynthesis-related genes was largely induced by salt stress. While genes encoding key proteins involved in photosynthesis were almost declined dramatically in -overexpression transgenic plants, and NaCl treatment further resulted in the down-regulation of these genes. Furthermore, DEGs encoding various plant hormone signal pathways were also identified. These results indicate that AeNAC83 is involved in resistance to salt stress and plant growth.
NAC 转录因子在植物生长、发育和应激反应中发挥着关键作用。此前，我们初步鉴定出转录因子 基因在黄秋葵中盐胁迫下显著上调（）。在此，我们从黄秋葵中克隆了核定位的 AeNAC83，并鉴定了其在盐胁迫反应和植物生长中的可能作用。通过病毒诱导的基因沉默下调 导致黄秋葵幼苗对盐胁迫的敏感性增强，生物量积累增加。同时，-过表达 系提高了耐盐性，并表现出许多改变的表型，包括小的莲座叶丛、短的主根，以及促进冠根和根毛的生长。RNA-seq 显示，在 -过表达转基因和野生型中，有许多基因在转录水平上发生了显著变化，无论是在有或没有 NaCl 处理的情况下。大多数苯丙烷和类黄酮生物合成相关基因的表达受到盐胁迫的强烈诱导。而参与光合作用的关键蛋白编码基因在 -过表达转基因植物中几乎急剧下降，NaCl 处理进一步导致这些基因的下调。此外，还鉴定了编码各种植物激素信号通路的 DEGs。这些结果表明，AeNAC83 参与了对盐胁迫的抗性和植物的生长。
