Aidos Lucia, Cafiso Alessandra, Lopez Annalaura, Vasconi Mauro, Valente Luisa M P, Bazzocchi Chiara, Di Giancamillo Alessia
Department of Biomedical Sciences for Health, University of Milan, 20133 Milan, Italy.
Department of Veterinary Medicine, University of Milan, 26900 Lodi, Italy.
Animals (Basel). 2022 Aug 26;12(17):2205. doi: 10.3390/ani12172205.
The aim of this study was to evaluate behaviour, growth, lipid composition, muscle development, and stress status of Siberian sturgeon larvae reared with two types of substrate: Bioballs1 (BB1) and Bioballs2 (BB2), when compared to no substrate (CTR). Sampling points were: hatching (T0), schooling (T1), and yolk-sac full absorption (T2). BB1 larvae were less active and showed no schooling behaviour. At T1 and at T2, BB1 larvae showed a significantly higher weight and total length than larvae reared in either CTR or BB2 (p < 0.05). The lipid content of larvae decreased over time, with little relevant differences between groups. At T2, total muscle area, slow muscle area and fast muscle area were significantly higher in larvae reared in BB1 (p < 0.05). No significant differences in muscle proliferation were found between groups. Real Time PCR was used for evaluating the relative expression of a pool of genes: myod, myog, mrf4, igf2, hsp70, hsp90a, hsp90b, and glut2. The expression of these genes did not seem to be much affected by the type of rearing substrate, except for myog and hsp70 at T1, which was greater in BB2 larvae. Our data suggest that the presence of a substrate during this developmental period seems to have positive effects but further studies would be necessary during the exogenous feeding stage.
本研究的目的是评估在两种类型的基质（生物球1（BB1）和生物球2（BB2））中饲养的西伯利亚鲟幼鱼的行为、生长、脂质组成、肌肉发育和应激状态，并与无基质（对照组）进行比较。采样点为：孵化期（T0）、集群期（T1）和卵黄囊完全吸收期（T2）。BB1幼鱼活跃度较低，未表现出集群行为。在T1和T2时，BB1幼鱼的体重和全长显著高于在对照组或BB2中饲养的幼鱼（p < 0.05）。幼鱼的脂质含量随时间下降，各组之间差异不大。在T2时，BB1中饲养的幼鱼的总肌肉面积、慢肌面积和快肌面积显著更高（p < 0.05）。各组之间在肌肉增殖方面未发现显著差异。使用实时荧光定量PCR评估一组基因的相对表达：肌分化抗原（myod）、肌细胞生成素（myog）、肌肉调节因子4（mrf4）、胰岛素样生长因子2（igf2）、热休克蛋白70（hsp70）、热休克蛋白90α（hsp90a）、热休克蛋白90β（hsp90b）和葡萄糖转运蛋白2（glut2）。这些基因的表达似乎受饲养基质类型的影响不大，除了在T1时的myog和hsp70，BB2幼鱼中的表达更高。我们的数据表明，在此发育阶段存在基质似乎具有积极影响，但在外源摄食阶段还需要进一步研究。
