Chen Rong, Yang Pengxia, Dai Zichun, Liu Jie, Zhu Huanxi, Lei Mingming, Shi Zhendan
Institute of Animal Science, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China.
Jiangsu Key Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base, Ministry of Science and Technology, Nanjing 210014, China.
Animals (Basel). 2022 Sep 2;12(17):2275. doi: 10.3390/ani12172275.
In order to explore the role of follistatin (FST) in ovarian follicular development and egg production in Yangzhou geese, sixty-four egg laying geese of the same genetic origin were selected and divided into two groups with equal numbers. One group was immunized against the recombinant goose FST protein by intramuscular injection, whereas the control group received bovine serum albumin (BSA) injection. Immunization against FST significantly increased the number of pre-ovulatory follicles. Furthermore, immunization against FST upregulated , , , , and mRNA levels in the granulosa layer of pre-hierarchical follicles. The results suggest that FST plays a limiting role in the development of ovarian pre-hierarchical follicles into pre-ovulatory follicles by decreasing follicular sensitivity to activin in geese. The mechanism may be achieved by regulating the SMAD3 signaling pathway, which affects progesterone synthesis and yolk deposition in pre-hierarchical follicles.
为了探究卵泡抑素（FST）在扬州鹅卵巢卵泡发育和产蛋中的作用，选取64只遗传背景相同的产蛋鹅，随机分为两组，每组数量相等。一组通过肌肉注射免疫重组鹅FST蛋白，而对照组注射牛血清白蛋白（BSA）。对FST进行免疫显著增加了排卵前卵泡的数量。此外，对FST进行免疫上调了前等级卵泡颗粒层中、、、和的mRNA水平。结果表明，FST通过降低鹅卵泡对激活素的敏感性，在卵巢前等级卵泡发育为排卵前卵泡的过程中发挥限制作用。其机制可能是通过调节SMAD3信号通路来实现的，该信号通路影响前等级卵泡中孕酮的合成和卵黄沉积。
