Sangboonruang Sirikwan, Semakul Natthawat, Manokruang Kiattikhun, Khammata Nuttawut, Jantakee Kanyaluck, Mai-Ngam Katanchalee, Charoenla Satrawut, Khamnoi Phadungkiat, Saengsawang Kanokwan, Wattananandkul Usanee, Intorasoot Sorasak, Tragoolpua Khajornsak
Division of Clinical Microbiology, Department of Medical Technology, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai 50200, Thailand.
Office of Research Administration, Chiang Mai University, Chiang Mai 50200, Thailand.
Int J Pharm X. 2024 Oct 11;8:100291. doi: 10.1016/j.ijpx.2024.100291. eCollection 2024 Dec.
Chronic wound infections are attributed to delayed tissue repair, which remains a major clinical challenge in long-term health care. Particularly, infections with antibiotic resistance have more serious effects on health, often resulting in unsuccessful treatments. Thus, antimicrobial peptide (AMP)-based therapy holds promise as a potential therapeutic approach to overcoming drug resistance. Conventional wound dressing is a passive strategy for wound care that is not capable of eradicating pathogens and promoting tissue repair. In this study, we aim to construct an advanced wound dressing; a thermo-responsive hydrogel incorporated with lactoferricin (Lfcin) niosome (Lfcin-Nio/hydrogel) for bacterial pathogen treatment. The Lfcin-loaded niosome (Lfcin-Nio) has a particle size of 396.91 ± 20.96 nm, 0.38 ± 0.01 of PdI, -10.5 ± 0.3 mV of ζ potential, and 72.30 ± 7.05 % Lfcin entrapment efficiency. Lfcin-Nio exhibited broad antibacterial activity on both drug-susceptible and drug-resistant strains, and also on bacteria residing in the biofilm matrix. The Lfcin-Nio/hydrogel was fabricated from 0.5 % / poloxamer 188-20 % w/v poloxamer 407, and supplemented with Lfcin-Nio and epidermal growth factor (EGF). The physical properties of Lfcin-Nio/hydrogels showed elasticity, swelling ability, and strong injectability with responsiveness to 33-37 °C temperatures. The biological properties of Lfcin-Nio/hydrogels exhibited a bactericidal effect against drug-resistant strains of and and showed less toxicity to the human skin fibroblast. It also promoted the healing of scratches by 55 % within 6 h, compared to the wound closure rate of 20 % in the cell control. The inflammatory response of the Lfcin-Nio/hydrogel-treated cells was reduced suppression of and mRNA expressions. From this study, Lfcin-Nio/hydrogels can be suggested as a modern wound dressing that possesses multifunctional and beneficial properties for the management of chronic wound infections.
慢性伤口感染归因于组织修复延迟，这在长期医疗保健中仍然是一个重大的临床挑战。特别是，具有抗生素耐药性的感染对健康有更严重的影响，常常导致治疗失败。因此，基于抗菌肽（AMP）的疗法有望成为克服耐药性的一种潜在治疗方法。传统的伤口敷料是一种被动的伤口护理策略，无法根除病原体和促进组织修复。在本研究中，我们旨在构建一种先进的伤口敷料；一种结合了乳铁蛋白（Lfcin）脂质体（Lfcin-Nio/水凝胶）的热响应水凝胶，用于治疗细菌病原体。负载Lfcin的脂质体（Lfcin-Nio）的粒径为396.91±20.96nm，多分散指数（PdI）为0.38±0.01，ζ电位为-10.5±0.3mV，Lfcin包封率为72.30±7.05%。Lfcin-Nio对药敏菌株和耐药菌株以及生物膜基质中的细菌均表现出广泛的抗菌活性。Lfcin-Nio/水凝胶由0.5%泊洛沙姆188-20%w/v泊洛沙姆407制成，并添加了Lfcin-Nio和表皮生长因子（EGF）。Lfcin-Nio/水凝胶的物理性质表现出弹性、膨胀能力以及在33-37°C温度下具有强烈的可注射响应性。Lfcin-Nio/水凝胶的生物学性质对耐甲氧西林金黄色葡萄球菌和耐万古霉素肠球菌的耐药菌株表现出杀菌作用，并且对人皮肤成纤维细胞的毒性较小。与细胞对照组20%的伤口闭合率相比，它还能在6小时内使划痕愈合率提高55%。Lfcin-Nio/水凝胶处理的细胞的炎症反应通过抑制白细胞介素-6和肿瘤坏死因子-α的mRNA表达而降低。从这项研究来看，Lfcin-Nio/水凝胶可被认为是一种对慢性伤口感染管理具有多功能和有益特性的现代伤口敷料。
