Masuda Y, Yoshitake Y, Nishikawa K
Cell Biol Int Rep. 1987 May;11(5):359-65. doi: 10.1016/0309-1651(87)90002-6.
A431 cells grew in protein-free Coon's modified Ham's F12 medium at a similar rate to that in medium supplemented with calf serum and secreted a growth factor capable of stimulating DNA synthesis in BALB/c3T3 cells. This factor had strong affinity for heparin and was partially purified from the conditioned medium by heparin-Sepharose affinity chromatography and molecular sieving on Bio-Gel P-60. The apparent molecular weight of the factor was 20-30K. Its activity was inhibited by heparin at concentrations of above 0.03 microgram/ml.
A431细胞在无蛋白的库恩改良哈姆氏F12培养基中生长的速率与在添加小牛血清的培养基中相似，并分泌一种能够刺激BALB/c3T3细胞DNA合成的生长因子。该因子对肝素具有很强的亲和力，并通过肝素-琼脂糖亲和层析和在Bio-Gel P-60上的分子筛从条件培养基中部分纯化。该因子的表观分子量为20 - 30K。其活性在浓度高于0.03微克/毫升时被肝素抑制。
