Yamada K, Yoshitake Y, Norimatsu H, Nishikawa K
Department of Biochemistry, Kanazawa Medical University, Ishikawa, Japan.
Cell Struct Funct. 1992 Feb;17(1):9-17. doi: 10.1247/csf.17.9.
The human osteosarcoma cell line (OST-1-PF) can grow in protein-free Coon's modified Ham's F12 medium. Growth of the cells in protein-free medium was partially density-dependent and partially depressed by medium change. An extract and conditioned medium of OST-1-PF cells contained high mitogenic activity for BALB/c3T3 cells. The growth factor in the cells was purified and identified as a basic fibroblast growth factor (bFGF)--like factor on the basis of its elution profile on heparin-affinity chromatography and the result of immunoblotting. An unidentified factor in a conditioned medium eliciting most of the DNA synthesis-stimulating activity showed a weak affinity for heparin. Various additions, including serum and growth factors, stimulated the growth of OST-1-PF cells in protein-free medium. Of these factors, epidermal growth factor (EGF), transforming growth factor-alpha (TGF-alpha), acidic fibroblast growth factor (aFGF) and bFGF were the most potent mitogens. High-affinity receptors of EGF and FGF were found on the surface of these cells. These results indicate that autonomous growth of OST-1-PF cells in protein-free medium is mainly controlled by an intracellular mechanism.
人骨肉瘤细胞系（OST-1-PF）可在无蛋白的库恩改良哈姆氏F12培养基中生长。细胞在无蛋白培养基中的生长部分依赖于密度，且培养基更换会使其生长受到部分抑制。OST-1-PF细胞的提取物和条件培养基对BALB/c3T3细胞具有高促有丝分裂活性。基于其在肝素亲和色谱上的洗脱图谱和免疫印迹结果，细胞中的生长因子被纯化并鉴定为一种碱性成纤维细胞生长因子（bFGF）样因子。条件培养基中引发大部分DNA合成刺激活性的一种未鉴定因子对肝素具有弱亲和力。包括血清和生长因子在内的各种添加物可刺激OST-1-PF细胞在无蛋白培养基中的生长。在这些因子中，表皮生长因子（EGF）、转化生长因子-α（TGF-α）、酸性成纤维细胞生长因子（aFGF）和bFGF是最有效的促有丝分裂原。在这些细胞表面发现了EGF和FGF的高亲和力受体。这些结果表明，OST-1-PF细胞在无蛋白培养基中的自主生长主要受细胞内机制控制。
