Masuda Y, Yoshitake Y, Nishikawa K
Department of Biochemistry, Kanazawa Medical University, Ishikawa, Japan.
In Vitro Cell Dev Biol. 1988 Sep;24(9):893-9. doi: 10.1007/BF02623899.
A431 cells grew at similar rates in protein-free Coon's modified Ham's F12 medium (PF-C-F12) with and without added bovine calf serum. The cells secreted a heparin-binding growth factor and a type-beta transforming growth factor, but their growth in PF-C-F12 was not affected by these factors, or by DNA synthesis factor from Rhodamine fibrosarcoma, basic fibroblast growth factor, insulin, human transferrin, bovine serum albumin, and their combinations. Growth of A431 cells in PF-C-F12 was not density dependent and was not affected by either addition of conditioned medium or replacement of conditioned medium by fresh medium. These results indicate that A431 cells have an intracellular mechanism for autonomous growth, and that their growth is not affected by factors that they secrete or by exogenous growth factors.
A431细胞在添加和未添加小牛血清的无蛋白库恩改良哈姆F12培养基（PF-C-F12）中生长速率相似。这些细胞分泌一种肝素结合生长因子和一种β型转化生长因子，但它们在PF-C-F12中的生长不受这些因子影响，也不受来自若丹明纤维肉瘤的DNA合成因子、碱性成纤维细胞生长因子、胰岛素、人转铁蛋白、牛血清白蛋白及其组合的影响。A431细胞在PF-C-F12中的生长不依赖密度，添加条件培养基或用新鲜培养基替换条件培养基均不影响其生长。这些结果表明，A431细胞具有自主生长的细胞内机制，其生长不受自身分泌因子或外源性生长因子的影响。
