Zhang Liping, Li Min, Fu Jianyu, Huang Xiaoqin, Yan Peng, Ge Shibei, Li Zhengzhen, Bai Peixian, Zhang Lan, Han Wenyan, Li Xin
Key Laboratory of Tea Quality and Safety Control, Ministry of Agriculture and Rural Affairs, Tea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou 310008, China.
College of Horticulture Science and Engineering, Shandong Agricultural University, Tai'an 271018, China.
Plants (Basel). 2022 Aug 29;11(17):2243. doi: 10.3390/plants11172243.
The tea plant is an important economic crop and is widely cultivated. Isopentenyl transferase (IPT) is the first and rate-limiting enzyme of cytokinin (CK) signaling, which plays key roles in plant development and abiotic stress. However, the gene family in tea plants has not been systematically investigated until now. The phylogenetic analyses, gene structures, and conserved domains were predicted here. The results showed that a total of 13 members were identified from a tea plant genome database and phylogenetically classified into four groups. Furthermore, 10 members belonged to plant ADP/ATP- genes, and 3 were tRNA- genes. There is a conserved putative ATP/GTP-binding site (P-loop motif) in all the CsIPT sequences. Based on publicly available transcriptome data as well as through RNA-seq and qRT-PCR analysis, the genes which play key roles in the development of different tissues were identified, respectively. Furthermore, may be involved in the response to different light treatments. may play a key role during the dormancy and flush of the lateral buds. may play important regulatory roles during the development of the lateral bud, leaf, and flower. and may both play key roles for increased resistance to cold-stress, whereas may play a key role in improving resistance to high-temperature stress as well as drought-stress and rewatering. This study could provide a reference for further studies of family's functions and could contribute to tea molecular breeding.
茶树是一种重要的经济作物，广泛种植。异戊烯基转移酶（IPT）是细胞分裂素（CK）信号传导的首个限速酶，在植物发育和非生物胁迫中起关键作用。然而，茶树中的该基因家族至今尚未得到系统研究。本文预测了其系统发育分析、基因结构和保守结构域。结果表明，从茶树基因组数据库中鉴定出总共13个成员，并在系统发育上分为四组。此外，10个成员属于植物ADP/ATP - 基因，3个是tRNA - 基因。所有CsIPT序列中都有一个保守的假定ATP/GTP结合位点（P环基序）。基于公开的转录组数据以及通过RNA测序和qRT - PCR分析，分别鉴定了在不同组织发育中起关键作用的基因。此外，可能参与对不同光照处理的响应。可能在侧芽的休眠和萌发过程中起关键作用。可能在侧芽、叶片和花朵的发育过程中起重要调节作用。和可能在增强抗寒胁迫方面都起关键作用，而可能在提高抗高温胁迫以及干旱胁迫和复水能力方面起关键作用。本研究可为进一步研究该家族的功能提供参考，并有助于茶树分子育种。
