Mohr Toni, Horstman James, Gu Yong Q, Elarabi Nagwa I, Abdallah Naglaa A, Thilmony Roger
USDA-ARS, Crop Improvement and Genetics Unit, Albany, CA 94710, USA.
Department of Genetics, Faculty of Agriculture, Cairo University, Giza 12613, Egypt.
Plants (Basel). 2022 Aug 30;11(17):2259. doi: 10.3390/plants11172259.
The highly conserved encodes a bifunctional enzyme with inositol polyphosphate-1-phosphatase and 3' (2'), 5'-bisphosphate nucleotidase activity and has been shown to alter abiotic stress tolerance in plants when disrupted. Precise gene editing techniques were used to generate mutants in hexaploid bread wheat. The CRISPR (Clustered Regulatory Interspaced Short Palindromic Repeats) Cas9 system with three guide RNAs (gRNAs) was used to inactivate six homologous genes within the Bobwhite wheat genome. The resulting mutant wheat plants with all their genes disabled had slimmer stems, had a modest reduction in biomass and senesced more slowly in water limiting conditions, but did not exhibit improved yield under drought conditions. Our results show that multiplexed gRNAs enabled effective targeted gene editing of the gene family in hexaploid wheat. These mutant wheat plants will be a resource for further research studying the function of this gene family in wheat.
高度保守的[基因名称]编码一种具有肌醇多磷酸-1-磷酸酶和3'（2'），5'-双磷酸核苷酸酶活性的双功能酶，并且已表明在其被破坏时会改变植物对非生物胁迫的耐受性。使用精确的基因编辑技术在六倍体面包小麦中产生[基因名称]突变体。具有三个引导RNA（gRNA）的CRISPR（成簇规律间隔短回文重复序列）Cas9系统用于使博白小麦基因组中的六个[基因名称]同源基因失活。所有[基因名称]基因均被破坏的所得突变小麦植株茎更细，生物量略有减少，并且在水分限制条件下衰老更慢，但在干旱条件下产量并未提高。我们的结果表明，多重gRNA能够对六倍体小麦中的[基因名称]基因家族进行有效的靶向基因编辑。这些[基因名称]突变小麦植株将成为进一步研究该基因家族在小麦中功能的资源。
