Chioti Vasileia, Zeliou Konstantina, Bakogianni Aikaterini, Papaioannou Charikleia, Biskinis Antonis, Petropoulos Constantinos, Lamari Fotini N, Papasotiropoulos Vasileios
Department of Agriculture, University of Patras, Nea Ktiria, 30200 Messolonghi, Greece.
Laboratory of Genetics, Department of Biology, University of Patras, 26504 Patras, Greece.
Plants (Basel). 2022 Aug 31;11(17):2267. doi: 10.3390/plants11172267.
Eggplant is a widely consumed vegetable, with significant nutritional value and high antioxidant content, mainly due to its phenolic constituents. Our goal was to determine the levels of carbohydrates, proteins, total phenolics, anthocyanins, flavonoids, chlorogenic acid, and the antioxidant capacity in thirteen eggplant cultivars cultivated in Greece and to identify sequence polymorphisms in key regulating genes of the phenylpropanoid pathway (, , , , , , ), which might relate to the phytochemical content of those cultivars. The carbohydrates' content differs among and within cultivars, while the rest of the phytochemicals differ only among cultivars. The cultivars 'EMI' and 'Lagkada' scored higher than the rest in phenolics, anthocyanins, ascorbic acid, caffeoylquinic acid, and antioxidant capacity. Moreover, significant correlations were observed between various ingredients and the antioxidant capacity (FRAP and DPPH). Sequence analysis revealed several SNPs in , , , , and among the cultivars studied. According to chi-square and logistic regression analyses, the missense mutation 4-108 correlates significantly with flavonoids, anthocyanins, and proteins; the synonymous mutation -105 correlates with anthocyanins and ascorbic acid; the missense mutation -438 correlates with flavonoids and chlorogenic acid, while the missense mutation 1-65 correlates with anthocyanins and sugars. These polymorphisms can be potentially utilized as molecular markers in eggplant breeding, while our data also contribute to the study of eggplant's secondary metabolism and antioxidant properties.
茄子是一种广泛食用的蔬菜，具有重要的营养价值和高抗氧化剂含量，这主要归因于其酚类成分。我们的目标是测定在希腊种植的13个茄子品种中的碳水化合物、蛋白质、总酚、花青素、黄酮类化合物、绿原酸水平以及抗氧化能力，并鉴定苯丙烷类途径关键调控基因（……）中的序列多态性，这些基因可能与这些品种的植物化学物质含量有关。碳水化合物含量在品种间和品种内存在差异，而其他植物化学物质仅在品种间存在差异。“EMI”和“Lagkada”品种在酚类、花青素、抗坏血酸、咖啡酰奎尼酸和抗氧化能力方面得分高于其他品种。此外，还观察到各种成分与抗氧化能力（FRAP和DPPH）之间存在显著相关性。序列分析揭示了在所研究的品种中，……等基因存在多个单核苷酸多态性（SNP）。根据卡方检验和逻辑回归分析，错义突变4 - 108与黄酮类化合物、花青素和蛋白质显著相关；同义突变 - 105与花青素和抗坏血酸相关；错义突变 - 438与黄酮类化合物和绿原酸相关，而错义突变1 - 65与花青素和糖类相关。这些多态性可潜在地用作茄子育种中的分子标记，同时我们的数据也有助于茄子次生代谢和抗氧化特性的研究。
