Key Laboratory of Biology and Germplasm Enhancement of Horticultural Crops in South China, Ministry of Agriculture, College of Horticulture, South China Agricultural University, Guangzhou 510642, China.
College of Horticulture, South China Agricultural University, Guangzhou 510642, China.
Int J Mol Sci. 2018 Mar 9;19(3):789. doi: 10.3390/ijms19030789.
Anthocyanins are the main pigments in flowers and fruits. These pigments are responsible for the red, red-purple, violet, and purple color in plants, and act as insect and animal attractants. In this study, phenotypic analysis of the purple flower color in eggplant indicated that the flower color is controlled by a single dominant gene, . Using an F₂ mapping population derived from a cross between purple-flowered 'Blacknite' and white-flowered 'Small Round', () was fine mapped to an approximately 165.6-kb region between InDel marker Indel8-11 and Cleaved Amplified Polymorphic Sequences (CAPS) marker Efc8-32 on Chromosome 8. On the basis of bioinformatic analysis, 29 genes were subsequently located in the target region, among which were two potential () gene candidates. Allelic sequence comparison results showed that one gene () was conserved in promoter and coding sequences without any nucleotide change between parents, whereas four single-nucleotide polymorphisms were detected in another gene (). Crucially, a single base pair deletion at site 438 resulted in premature termination of , leading to the loss of anthocyanin accumulation. In addition, displayed strong expression in purple flowers compared with white flowers and other tissues. Collectively, our results indicate that is a good candidate gene for , which controls anthocyanidin synthase in eggplant flowers. The present study provides information for further potential facilitate genetic engineering for improvement of anthocyanin levels in plants.
花色苷是花和果实中的主要色素。这些色素使植物呈现出红色、红紫色、紫色和紫红色，并且作为昆虫和动物的引诱剂。在这项研究中，对茄子紫色花色的表型分析表明，花色由一个单一的显性基因控制，。利用来自紫色花“黑美人”和白花“小圆茄”杂交的 F₂ 作图群体，将（）精细定位到 8 号染色体 InDel 标记 Indel8-11 和 Cleaved Amplified Polymorphic Sequences (CAPS)标记 Efc8-32 之间约 165.6-kb 的区域。基于生物信息学分析，随后在 29 个基因中定位到 目标区域，其中包括两个潜在的（）基因候选物。等位基因序列比较结果表明，一个（）基因（）在启动子和编码序列中与亲本保守，没有核苷酸变化，而另一个（）基因在另一个基因（）中检测到四个单核苷酸多态性。关键的是，在第 438 位的单个碱基对缺失导致提前终止，导致花色苷积累的丧失。此外，与白花和其他组织相比，在紫色花中（）表现出强烈的表达。综上所述，我们的结果表明，是茄子花色的候选基因，它控制花色苷合成酶。本研究为进一步的潜在遗传工程提供了信息，以提高植物中花色苷的水平。
