Royal College of Surgeons in Ireland Bahrain, Adliya 15503, Bahrain.
Academic Endocrinology, Diabetes and Metabolism, Hull York Medical School, Hull YO10 5DD, UK.
Nutrients. 2022 Aug 27;14(17):3540. doi: 10.3390/nu14173540.
Chronic low-grade inflammation is a characteristic of women with polycystic ovary syndrome (PCOS), although this may be obesity-driven rather than an intrinsic facet of PCOS; furthermore, vitamin D deficiency, another common feature of PCOS, is reported to have an association with increased inflammation. Therefore, circulating inflammatory protein levels and circulating levels of vitamin D may be linked in PCOS, though it is unclear which vitamin D metabolites may be important.
We measured plasma levels of 24 inflammatory proteins and 12 matrix metalloproteinases (proteins modulated by the inflammatory process) by slow off-rate modified aptamer (SOMA)-scan plasma protein measurement in weight and aged-matched non-obese non-insulin resistant PCOS (n = 24) and control (n = 24) women. Inflammatory proteins and matrix metalloproteinases were correlated to 25-hydroxy vitamin D (25(OH)D), its epimer 25-hydroxy-3epi-vitamin D (3epi25(OH)D) and the active 1,25-dihydroxyvitamin D (1,25(OH)D) as measured by gold standard isotope-dilution liquid chromatography tandem mass spectrometry.
PCOS women had both an elevated free androgen index and circulating anti-mullerian hormone, though insulin resistance was comparable to controls. C-reactive protein, as a standard circulatory marker of inflammation, was comparable between cohorts. Levels of circulating inflammatory proteins and matrix metalloproteinases were not different between the PCOS and control women, with no correlation of 25(OH)D 1,25(OH)D or 3epi25(OH)D with any of the inflammatory proteins.
In a non-obese PCOS population matched for age and insulin resistance, circulating inflammatory proteins and matrix metalloproteinases were not elevated and did not correlate with 25(OH)D its epimer 3epi25(OH)D or 1,25(OH)D in either control or PCOS women, indicating that the inflammatory response is absent and the vitamin D-metabolite independent in non-obese women with PCOS.
慢性低度炎症是多囊卵巢综合征（PCOS）女性的特征，尽管这可能是肥胖驱动的，而不是 PCOS 的固有特征；此外，维生素 D 缺乏是 PCOS 的另一个常见特征，据报道与炎症增加有关。因此，循环炎症蛋白水平和循环维生素 D 水平可能与 PCOS 有关，但尚不清楚哪些维生素 D 代谢物可能很重要。
我们通过慢脱靶修饰适体（SOMA）扫描血浆蛋白测量法测量了体重和年龄匹配的非肥胖非胰岛素抵抗 PCOS（n=24）和对照组（n=24）女性的 24 种炎症蛋白和 12 种基质金属蛋白酶（受炎症过程调节的蛋白质）的血浆水平。将炎症蛋白和基质金属蛋白酶与 25-羟维生素 D（25（OH）D）、其表异构 25-羟-3-表维生素 D（3epi25（OH）D）和活性 1,25-二羟维生素 D（1,25（OH）D）相关联，这些物质由金标准同位素稀释液相色谱串联质谱法测量。
PCOS 女性的游离雄激素指数和循环抗苗勒管激素均升高，尽管胰岛素抵抗与对照组相当。C 反应蛋白作为炎症的标准循环标志物，在两组之间无差异。循环炎症蛋白和基质金属蛋白酶水平在 PCOS 女性和对照组女性之间无差异，25（OH）D、1,25（OH）D 或 3epi25（OH）D 与任何一种炎症蛋白均无相关性。
在年龄和胰岛素抵抗相匹配的非肥胖 PCOS 人群中，循环炎症蛋白和基质金属蛋白酶并未升高，并且在对照组或 PCOS 女性中均与 25（OH）D 及其表异构 3epi25（OH）D 或 1,25（OH）D 无关，这表明非肥胖的 PCOS 女性中不存在炎症反应，且不受维生素 D 代谢物的影响。
