Popov Anton L, Zholobak Nadia M, Shcherbakov Alexander B, Kozlova Taisiya O, Kolmanovich Danil D, Ermakov Artem M, Popova Nelli R, Chukavin Nikita N, Bazikyan Ernest A, Ivanov Vladimir K
Kurnakov Institute of General and Inorganic Chemistry of the Russian Academy of Sciences, Moscow 119991, Russia.
Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino 142290, Moscow Region, Russia.
Nanomaterials (Basel). 2022 Sep 1;12(17):3034. doi: 10.3390/nano12173034.
We studied the toxic effects of cerium and fluoride species on human dental pulp stem cells and epithelial cells of as a surrogate for the human oral mucosa. The sequential use of CeCl and NHF solutions in equimolar sub-toxic concentrations enabled the possible toxic effects of individual components to be avoided, ensuring the preservation of the metabolic activity of the cells due to the formation of CeF nanoparticles. Cerium fluoride nanoparticles and terbium-doped cerium fluoride nanoparticles exhibited neither cytotoxicity nor genotoxicity to dental pulp stem cells, even at high concentrations (10 M). In millimolar concentrations (from 10-10 M), these nanoparticles significantly increased the expression of genes responsible for the cell cycle, differentiation and proliferation. The formation of cerium fluoride on the surface of the mucous membrane and teeth provided protection against the development of carious lesions, periodontitis, ROS attacks and other inflammatory diseases of the oral cavity. Luminescent CeF: Tb nanoparticles enabled the visualization of tooth enamel microcracks.
我们研究了铈和氟化物对人牙髓干细胞及作为人口腔黏膜替代物的上皮细胞的毒性作用。以等摩尔亚毒性浓度依次使用CeCl和NHF溶液，能够避免单个成分可能产生的毒性作用，由于形成了CeF纳米颗粒，确保了细胞代谢活性的保留。氟化铈纳米颗粒和掺铽氟化铈纳米颗粒对牙髓干细胞既无细胞毒性也无基因毒性，即使在高浓度（10 M）时也是如此。在毫摩尔浓度（从10⁻¹⁰ M）下，这些纳米颗粒显著增加了负责细胞周期、分化和增殖的基因的表达。在黏膜和牙齿表面形成氟化铈可预防龋齿、牙周炎、活性氧攻击及口腔其他炎症性疾病的发生。发光的CeF:Tb纳米颗粒能够使牙釉质微裂纹可视化。
