Department of Animal Physiology, Biochemistry and Biostructure, Faculty of Veterinary Medicine and Animal Sciences, Poznań University of Life Sciences, Wołyńska 35, 60-637 Poznań, Poland.
Department of Human Nutrition and Dietetics, Poznań University of Life Sciences, Wojska Polskiego 31, 60-624 Poznań, Poland.
Molecules. 2022 Aug 29;27(17):5568. doi: 10.3390/molecules27175568.
Oxidative stress and inflammation play a crucial role in the pathogenesis and progression of diabetes. Currently, there is a growing need to exploit plant-derived bioactive compounds to support conventional therapies. The purpose of this study was to explore allyl isothiocyanate (AITC) potency in reducing oxidative and inflammatory stress along with its profitable modulation trace element status in pathological conditions such as diabetes. Two weeks of oral AITC treatments (2.5, 5, and 25 mg/kg body weight per day) were evaluated in Wistar rats with diabetes induced by a high-fat diet and streptozotocin. The study included AITC influence on antioxidant factors (SOD, CAT, GST, Nrf2), stress and inflammatory markers (cortisol, CRP, IL-1β, IL-6, TNFα, NF-κB), lipid peroxidation indices (TBARS, -SH groups), and trace element status (Fe, Zn, and Cu) in the detoxification and lymphoid organs. Independently of dose, AITC increased cortisol levels in rat blood serum and decreased total thiol groups (T-SH) and protein-bound thiol groups (PB-SH) collaterally with raised thiobarbituric acid reactive substances (TBARS) in diabetic rat liver. The inflammation and oxidative effects were enhanced by an AITC dose increase. The highest dose of AITC, 25 mg/kg b.w., strongly affected the inflammation process by increasing IL-6, IL-1β, and TNFα in the blood serum, and it upregulated Nrf2 transcription factor with increased SOD, GPx, and GST activities in the liver. AITC showed an equivocal effect on profitable modulation of disturbances in mineral homeostasis in the liver, kidney, and spleen. Our findings revealed that two-week AITC treatment exacerbated oxidative and inflammation status in diabetic rats.
氧化应激和炎症在糖尿病的发病机制和进展中起着至关重要的作用。目前，越来越需要利用植物源性生物活性化合物来支持传统疗法。本研究旨在探讨丙烯基异硫氰酸酯 (AITC) 在降低氧化和炎症应激方面的效力，以及其在糖尿病等病理情况下对微量元素状态的有益调节作用。在高脂肪饮食和链脲佐菌素诱导的糖尿病 Wistar 大鼠中，评估了两周的口服 AITC 治疗（每天 2.5、5 和 25mg/kg 体重）。该研究包括 AITC 对抗氧化因子（SOD、CAT、GST、Nrf2）、应激和炎症标志物（皮质醇、CRP、IL-1β、IL-6、TNFα、NF-κB）、脂质过氧化指数（TBARS、-SH 基团）和解毒和淋巴器官中的微量元素状态（Fe、Zn 和 Cu）的影响。独立于剂量，AITC 增加了糖尿病大鼠血清中的皮质醇水平，并降低了总巯基基团（T-SH）和蛋白结合巯基基团（PB-SH），同时伴有肝中硫代巴比妥酸反应物质（TBARS）升高。随着 AITC 剂量的增加，炎症和氧化作用增强。AITC 的最高剂量 25mg/kg b.w. 通过增加血清中的 IL-6、IL-1β 和 TNFα，强烈影响炎症过程，并通过增加 SOD、GPx 和 GST 活性来上调 Nrf2 转录因子。AITC 对肝脏、肾脏和脾脏中矿物质内稳态紊乱的有利调节作用表现出不确定的效果。我们的研究结果表明，两周的 AITC 治疗加剧了糖尿病大鼠的氧化和炎症状态。
