Pediatric Department, Medical College, Najran University, Najran 55461, Saudi Arabia.
Department of Pharmaceutical Chemistry, College of Pharmacy, Najran University, Najran 55461, Saudi Arabia.
Molecules. 2022 May 26;27(11):3444. doi: 10.3390/molecules27113444.
Diabetes mellitus is a metabolic disorder and is a global challenge to the current medicinal chemists and pharmacologists. This research has been designed to isolate and evaluate antidiabetic bioactives from . The crude extracts, semi-purified and pure bioactives have been used in all in vitro assays. The in vitro α-glucosidase, α-amylase and DPPH free radical activities have been performed on all plant samples. The initial activities showed that ethyl acetate () was the potent fraction in all the assays. This fraction was initially semi-purified to obtain -. Among the semi-purified fractions, was dominant, exhibiting potent IC values in all the in vitro assays. Based on the potency and availability of materials, was subjected to further purification to obtain compounds (2,4-dichloro-6-hydroxy-3,5-dimethoxytoluene) and (2-methyl-6-(4-methylphenyl)-2-hepten-4-one). The two isolated compounds were characterized by mass and NMR analyses. The compounds and showed excellent inhibitions against α-glucosidase (21.45 for and 15.03 for μg/mL), α-amylase (17.65 and 16.56 μg/mL) and DPPH free radicals (7.62 and 14.30 μg/mL). Our study provides baseline research for the antidiabetic bioactives exploration from . The bioactive compounds can be evaluated in animals-based antidiabetic activity in future.
糖尿病是一种代谢紊乱，是当前医学化学家和药理学家面临的全球性挑战。本研究旨在从 中分离和评估抗糖尿病生物活性成分。所有体外试验均使用粗提物、半纯化和纯生物活性成分。已对所有植物样品进行体外α-葡萄糖苷酶、α-淀粉酶和 DPPH 自由基活性测定。初步活性表明，乙酸乙酯（）在所有测定中是最有效的部分。该部分最初被半纯化以获得 。在半纯化的部分中， 占主导地位，在所有体外试验中均表现出很强的 IC 值。基于材料的功效和可得性， 进一步纯化以获得化合物 （2,4-二氯-6-羟基-3,5-二甲氧基甲苯）和 （2-甲基-6-（4-甲基苯基）-2-庚烯-4-酮）。这两种分离出的化合物通过质谱和 NMR 分析进行了表征。化合物 和 对α-葡萄糖苷酶（ 21.45 对 和 15.03 对 μg/mL）、α-淀粉酶（17.65 和 16.56 μg/mL）和 DPPH 自由基（7.62 和 14.30 μg/mL）具有极好的抑制作用。我们的研究为从 中探索抗糖尿病生物活性成分提供了基础研究。将来可以在动物抗糖尿病活性模型中评估这些生物活性化合物。
