Department of Microbiology and Clinical Immunology, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Oczapowskiego Street 13, 10-719 Olsztyn, Poland.
Molecules. 2022 Aug 29;27(17):5569. doi: 10.3390/molecules27175569.
Enterovirus E (EV-E), a representative of the Picornaviridae family, endemically affects cattle across the world, typically causing subclinical infections. However, under favorable conditions, severe or fatal disorders of the respiratory, digestive, and reproductive systems may develop. There is no specific treatment for enterovirus infections in humans or animals, and only symptomatic treatment is available. The aim of this study was to determine the in vitro antiviral effect of bovine lactoferrin (bLF) against enterovirus E using virucidal, cytopathic effect inhibition, and viral yield reduction assays in MDBK cells. The influence of lactoferrin on the intracellular viral RNA level was also determined. Surprisingly, lactoferrin did not have a protective effect on cells, although it inhibited the replication of the virus during the adsorption and post-adsorption stages (viral titres reduced by 1-1.1 log). Additionally, a decrease in the viral RNA level in cells (by up to 75%) was observed. More detailed studies are needed to determine the mechanism of bovine lactoferrin effect on enterovirus E. However, this highly biocompatible protein ensures some degree of protection against infection by bovine enterovirus, which is particularly important for young animals that receive this protein in their mother's milk.
肠病毒 E（EV-E）是小核糖核酸病毒科的代表病毒，在全球范围内地方性地感染牛，通常引起亚临床感染。然而，在有利条件下，可能会发展为严重或致命的呼吸系统、消化系统和生殖系统疾病。人类或动物的肠病毒感染没有特定的治疗方法，只有对症治疗。本研究旨在使用 MDBK 细胞中的病毒杀灭、细胞病变效应抑制和病毒产量减少测定法，确定牛乳铁蛋白（bLF）对肠病毒 E 的体外抗病毒作用。还确定了乳铁蛋白对细胞内病毒 RNA 水平的影响。令人惊讶的是，乳铁蛋白虽然在吸附和吸附后阶段抑制了病毒的复制（病毒滴度降低了 1-1.1 对数），但对细胞没有保护作用。此外，还观察到细胞中的病毒 RNA 水平下降（最多达 75%）。需要进行更详细的研究以确定牛乳铁蛋白对肠病毒 E 作用的机制。然而，这种高度生物相容的蛋白质确保了对牛肠病毒感染的一定程度的保护，这对于从母亲的乳汁中获得这种蛋白质的幼小动物尤为重要。
