Lin Tzou-Yien, Chu Chishih, Chiu Cheng-Hsun
Department of Pediatrics, Chang Gung Children's Hospital, 5 Fu-Hsin Street, Kweishan 333, Taoyuan, Taiwan.
J Infect Dis. 2002 Oct 15;186(8):1161-4. doi: 10.1086/343809. Epub 2002 Sep 16.
Enterovirus 71 (EV71), the newest member of Enterovirudae, is notable for its etiological role in epidemics of severe neurological diseases in children. It appears to be emerging as an important virulent neurotropic enterovirus in the upcoming era of poliomyelitis eradication, whereas no effective vaccine or antiviral agents are available at this moment. Human and bovine lactoferrins, iron-binding proteins belonging to the nonimmune defense system, were assayed in vitro to assess their inhibiting capacity on the cytopathic effect of EV71 on human embryonal rhabdomyosarcoma (RD) cells. Both bovine and human lactoferrins were found to be potent inhibitors of EV71 infection (mean IC(50), 10.5-24.5 microg/mL and 103.3-185.0 microg/mL, respectively). Lactoferrin probably exerts its effect at the level of viral adsorption, since the ongoing infection could not be further inhibited after the EV71 penetrated RD cells.
肠道病毒71型（EV71）是小RNA病毒科的最新成员，因其在儿童严重神经疾病流行中的病因学作用而备受关注。在即将根除脊髓灰质炎的时代，它似乎正成为一种重要的嗜神经毒性肠道病毒，而目前尚无有效的疫苗或抗病毒药物。人乳铁蛋白和牛乳铁蛋白属于非免疫防御系统的铁结合蛋白，在体外进行检测以评估它们对EV71对人胚胎横纹肌肉瘤（RD）细胞的细胞病变效应的抑制能力。发现牛乳铁蛋白和人乳铁蛋白都是EV71感染的有效抑制剂（平均半数抑制浓度分别为10.5 - 24.5微克/毫升和103.3 - 185.0微克/毫升）。乳铁蛋白可能在病毒吸附水平发挥作用，因为在EV71穿透RD细胞后，持续感染无法进一步被抑制。
