Feng Guidan, Kang Taisheng, Tang Pan, Xie Caihua, Yan Ruoqian, Qian Weidong
Shanghai Animal Disease Prevention and Control Center, Shanghai 201103, China.
Institute of Animal Husbandry and Veterinary Medicine, Shanghai Academy of Agricultural Sciences, Shanghai 201106, China.
Animals (Basel). 2025 May 18;15(10):1457. doi: 10.3390/ani15101457.
Bovine enteroviruses (BEVs) are emerging pathogens with poorly understood evolutionary dynamics and zoonotic potential. Here, we report the discovery of a novel recombinant BEV strain, HeN-2022, isolated from cattle in China. Genomic analysis revealed that HeN-2022 is a primary hybrid of BEV-E1 (VG527, Ireland) and BEV-E4 (GX1901, China), with recombination breakpoints in the VP1 gene and 5' UTR. Divergence dating traced its origin to 1991, predating closely related strains. Experimental infection in sheep demonstrated asymptomatic viral shedding (peak at 5 dpi) and robust neutralizing antibody responses, highlighting the potential cross-species adaptability. These findings underscore recombination as a potential key driver of BEV evolution and emphasize the need for global surveillance to address emerging livestock pathogens.
牛肠道病毒（BEVs）是一类新兴病原体，其进化动态和人畜共患病潜力尚不清楚。在此，我们报告了从中国牛群中分离出的一种新型重组牛肠道病毒毒株HeN-2022的发现。基因组分析表明，HeN-2022是BEV-E1（VG527，爱尔兰）和BEV-E4（GX1901，中国）的主要杂交毒株，在VP1基因和5'UTR中有重组断点。分歧年代测定将其起源追溯到1991年，早于密切相关的毒株。对绵羊的实验性感染显示出无症状的病毒脱落（在感染后第5天达到峰值）和强烈的中和抗体反应，突出了潜在的跨物种适应性。这些发现强调了重组是牛肠道病毒进化的一个潜在关键驱动因素，并强调了全球监测以应对新兴家畜病原体的必要性。
