Frixen U, Yamasaki H
Carcinogenesis. 1987 Aug;8(8):1101-4. doi: 10.1093/carcin/8.8.1101.
Tumour-promoting phorbol esters and diacylglycerols have a variety of biochemical and biological effects in common in a number of cell culture systems; thus, it has been suggested that diacylglycerols are endogenous functional analogues of phorbol esters. In order to examine more directly the possible relevance of these functional analogues in tumour promotion, we have studied the promoting effect of 1-oleoyl-2-acetyl glycerol (OAG) in the two-stage transformation model with BALB/c 3T3 cells, which is designed to simulate in vivo two-stage carcinogenesis. When BALB/c 3T3 cells were treated once with a low dose of 3-methylcholanthrene (MCA), almost no increase in cell transformation occurred. However, repeated application of OAG after MCA treatment enhanced cell transformation. OAG enhanced transformation to a lesser extent than a potent promoter of in vivo and in vitro carcinogenesis, phorbol-12,13-didecanoate. OAG also continuously inhibited junctional intercellular communication when BALB/c 3T3 cells were treated daily at confluence. These results indicate that diacylglycerols mimic phorbol esters in promoting cell transformation and that the enhancement may be related to an inhibitory effect on intercellular communication.
促肿瘤佛波酯和二酰基甘油在许多细胞培养系统中具有多种共同的生化和生物学效应；因此，有人提出二酰基甘油是佛波酯的内源性功能类似物。为了更直接地研究这些功能类似物在肿瘤促进中的可能相关性，我们在BALB/c 3T3细胞的两阶段转化模型中研究了1-油酰基-2-乙酰甘油（OAG）的促进作用，该模型旨在模拟体内两阶段致癌过程。当用低剂量的3-甲基胆蒽（MCA）处理一次BALB/c 3T3细胞时，细胞转化几乎没有增加。然而，MCA处理后重复应用OAG可增强细胞转化。与体内和体外致癌的强效促进剂佛波醇-12,13-二癸酸酯相比，OAG增强转化的程度较小。当BALB/c 3T3细胞在汇合时每天进行处理，OAG还持续抑制细胞间连接通讯。这些结果表明，二酰基甘油在促进细胞转化方面模拟了佛波酯，并且这种增强可能与对细胞间通讯的抑制作用有关。
