一种基于新型导电纳米复合材料的生物传感器，用于以亚甲蓝为媒介物超灵敏检测血清中的微小RNA-21。

A novel conductive nanocomposite-based biosensor for ultrasensitive detection of microRNA-21 in serum, using methylene blue as mediator.

作者信息

Deng Mengtian, Feng Jialu, Tao Dan, Yan Hanhui, Ding Jingjing, Jaffrezic-Renault Nicole, Guo Zhenzhong

机构信息

Hubei Province Key Laboratory of Occupational Hazard identification and Control, Wuhan University of Science and Technology, Wuhan 430065, China; School of Public Health, Medical College, Wuhan University of Science and Technology, Wuhan 430065, China.

Hubei Province Key Laboratory of Occupational Hazard identification and Control, Wuhan University of Science and Technology, Wuhan 430065, China.

出版信息

Bioelectrochemistry. 2022 Dec;148:108256. doi: 10.1016/j.bioelechem.2022.108256. Epub 2022 Sep 5.

DOI:10.1016/j.bioelechem.2022.108256

PMID:36081272

Abstract

MicroRNA-21 (miRNA-21) is a common biomarker with high expression in breast tumors. Therefore, sensitive detection of miRNA-21 is of great significance for clinical breast tumor diagnosis. A TH/rGO/CMK-3/AuNPs nanocomposite is composed of thionine (TH), reduced graphene oxide (rGO), ordered mesoporous carbon (CMK-3), and gold nanoparticles (AuNPs), which help to increase the specific surface area of a glassy carbon electrode (GCE) and to amplify the DPV signal. Meanwhile, methylene blue (MB) was combined with the capture probe guanine and absorbed by the composite material to mediate the differential pulse voltammetry (DPV) of the obtained miRNA biosensor. The current response decreased with increasing miRNA-21 concentration under optimal conditions. The biosensor responds to miRNA-21 in the 0.1fM-1 pM concentration range, and the detection limit (LOD) was 0.046 fM. Moreover, human serum samples were effectively detected utilizing the miRNA-21 biosensor with satisfactory results.

摘要

微小RNA-21（miRNA-21）是一种在乳腺肿瘤中高表达的常见生物标志物。因此，灵敏检测miRNA-21对临床乳腺肿瘤诊断具有重要意义。一种硫堇（TH）/还原氧化石墨烯（rGO）/有序介孔碳（CMK-3）/金纳米颗粒（AuNPs）纳米复合材料由硫堇（TH）、还原氧化石墨烯（rGO）、有序介孔碳（CMK-3）和金纳米颗粒（AuNPs）组成，有助于增加玻碳电极（GCE）的比表面积并放大差分脉冲伏安法（DPV）信号。同时，亚甲基蓝（MB）与捕获探针鸟嘌呤结合并被复合材料吸收，以介导所得miRNA生物传感器的差分脉冲伏安法（DPV）。在最佳条件下，电流响应随miRNA-21浓度的增加而降低。该生物传感器在0.1fM - 1 pM浓度范围内对miRNA-21有响应，检测限（LOD）为0.046 fM。此外，利用miRNA-21生物传感器对人血清样本进行了有效检测，并获得了满意的结果。