Hubei Province Key Laboratory of Occupational Hazard Identification and Control, Wuhan University of Science and Technology, Wuhan, 430065, People's Republic of China.
School of Public Health, Medical College, Wuhan University of Science and Technology, Wuhan, 430065, People's Republic of China.
Anal Bioanal Chem. 2022 Oct;414(24):7325-7334. doi: 10.1007/s00216-022-04284-0. Epub 2022 Aug 16.
Achieving the early diagnosis of breast cancer, through ultrasensitive detection of tumor marker miRNA-155, is a significant challenge. Therefore, an ultrasensitive hairpin electrochemical biosensor based on graphite-like phase carbon nitride composite was proposed. In this paper, poly(D-glucosamine) (PDG) was used as a stabilizer and reducing agent to prepare gold nanoparticles at room temperature, and then a graphite-like phase with a two-dimensional lamellar structure carbon nitride was further combined with it to obtain the poly(D-glucosamine)/gold nanoparticles/graphite-like phase carbon nitride nanocomposite (PDG/AuNPs/g-CN), in order to achieve the goal of signal amplification. The specific hairpin capture probe (HP) that recognized and bound miRNA-155 was then grafted. The hairpin biosensor showed a linear range of 0.1 fM-1 pM with a detection limit of 0.05 fM using differential pulse voltammetry (DPV) electrochemical analysis. Furthermore, the excellent performance hairpin electrochemical biosensor had been applied to the detection of miRNA-155 in human serum samples with good recovery.
实现乳腺癌的早期诊断，通过超灵敏检测肿瘤标志物 miRNA-155，是一个重大的挑战。因此，提出了一种基于石墨相氮化碳复合材料的超灵敏发夹电化学生物传感器。在本文中，聚（D-葡萄糖胺）（PDG）被用作稳定剂和还原剂，在室温下制备金纳米粒子，然后进一步将其与具有二维层状结构的石墨相氮化碳结合，得到聚（D-葡萄糖胺）/金纳米粒子/石墨相氮化碳纳米复合材料（PDG/AuNPs/g-CN），以实现信号放大的目的。然后接枝了特异性识别和结合 miRNA-155 的发夹捕获探针（HP）。使用差分脉冲伏安法（DPV）电化学分析，发夹生物传感器表现出 0.1 fM-1 pM 的线性范围和 0.05 fM 的检测限。此外，该超灵敏发夹电化学生物传感器已应用于人血清样品中 miRNA-155 的检测，具有良好的回收率。
