Parnham A J, Tarbit I F
Clin Chem. 1987 Aug;33(8):1421-4.
We assessed the LKB "Delfia" (time-resolved dissociation-enhanced lanthanide fluoroimmunoassay) and the Amersham "Amerlite" (enhanced luminescent immunometry) assays of thyrotropin in serum. Both assays are sensitive (respective detection limits: 0.02 and 0.04 milli-int. unit/L) and have very good within- and between-batch precision over a wide range of thyrotropin concentrations. Results by the two methods correlate well (r = 0.992); the regression equation is: Amerlite = 0.915 Delfia - 0.33 milli-int. unit/L. The standard curve for the Delfia assay was linear, but that for the Amerlite assay showed some deviation from linearity below 0.5 milli-int. unit/L. Both assays have a negative bias in comparison with radiolabeled immunoradiometric assays, as judged by results for samples from the Quality Assurance Scheme. Both assays discriminate well between hyper-, hypo-, and euthyroid subjects, and results for thyrotropin for most patients with nonthyroidal illness were within the euthyroid reference interval. Both assays are convenient to perform and are based on systems that provide a viable alternative to radioimmunoassay.
我们评估了血清促甲状腺激素的LKB“Delfia”（时间分辨解离增强镧系元素荧光免疫分析）和Amersham“Amerlite”（增强型发光免疫分析）检测方法。两种检测方法均灵敏（各自的检测限分别为0.02和0.04毫国际单位/升），并且在很宽的促甲状腺激素浓度范围内，批内和批间精密度都非常好。两种方法的结果相关性良好（r = 0.992）；回归方程为：Amerlite = 0.915 Delfia - 0.33毫国际单位/升。Delfia检测的标准曲线呈线性，但Amerlite检测的标准曲线在低于0.5毫国际单位/升时显示出一定程度的线性偏差。根据质量保证计划样本的结果判断，与放射性标记免疫放射分析相比，两种检测方法均存在负偏差。两种检测方法在区分甲状腺功能亢进、减退和正常的受试者方面表现良好，大多数非甲状腺疾病患者的促甲状腺激素结果都在甲状腺功能正常的参考区间内。两种检测方法操作方便，且基于能为放射免疫分析提供可行替代方案的系统。
