Helenius T, Tikanoja S
Clin Chem. 1986 Mar;32(3):514-8.
We describe a two-site immunoradiometric assay for thyrotropin (TSH) in serum, based on use of two monoclonal antibodies directed against two separate antigenic determinants on the TSH molecule. One antibody is immobilized on polystyrene beads; the other is radioiodinated by a modified Chloramine T method. The detection limit of the assay is 0.02 milli-int. unit/L. The working range (CV less than 10%) is from 0.1 to greater than 50 milli-int. units/L. The log mean concentration of TSH in sera collected from 100 euthyroid subjects between 08:00 and 11:00 hours was 1.9 milli-int. units/L, the range 0.4-5.4 milli-int. units/L. Values for hyperthyroid patients and thyroid-cancer patients being treated with thyroxin were much lower than those for euthyroid persons. Results by this new assay correlated excellently with those by our conventional radioimmunoassay (r = 0.99) and also with a sensitive immunofluorometric TSH method (Delfia TSH) (r = 0.99).
我们描述了一种用于检测血清中促甲状腺激素（TSH）的双位点免疫放射分析方法，该方法基于使用两种针对TSH分子上两个不同抗原决定簇的单克隆抗体。一种抗体固定在聚苯乙烯珠上；另一种通过改良的氯胺T法进行放射性碘化。该分析方法的检测限为0.02毫国际单位/升。工作范围（变异系数小于10%）为0.1至大于50毫国际单位/升。从100名甲状腺功能正常的受试者在8:00至11:00之间采集的血清中TSH的对数平均浓度为1.9毫国际单位/升，范围为0.4 - 5.4毫国际单位/升。甲状腺功能亢进患者和接受甲状腺素治疗的甲状腺癌患者的值远低于甲状腺功能正常者。这种新分析方法的结果与我们传统的放射免疫分析结果（r = 0.99）以及灵敏的免疫荧光TSH方法（Delfia TSH）结果（r = 0.99）相关性极佳。
