Development of a loop-mediated isothermal amplification assay for the rapid detection of and .

is the only deadly species in the genus with a mortality rate of more than 50%, and is the most common poisonous species, making rapid species identification in mushroom poisoning incidents extremely important. The main objective of this study was to develop a rapid, specific, sensitive, and simple loop-mediated isothermal amplification (LAMP) assay for the detection of and . Two sets of species-specific LAMP primers targeting internal transcribed spacer (ITS) regions were designed to identify and . The results demonstrated that while LAMP could specifically detect and , the polymerase chain reaction (PCR) could not distinguish from . In addition, the results demonstrated that, compared to electrophoresis-LAMP and real-time quantitative LAMP (RT-qLAMP), the detection sensitivity of HNB-LAMP (a mixture of LAMP with hydroxy naphthol blue (HNB) dye) for could reach 0.5 pg/μl and was 100-fold higher than that of PCR. The LAMP reaction could be completed in 45 min, which is much faster than the conventional PCR. In the future, LAMP can be used a quick, specific, and sensitive detection tool in various fields.