Narayanaperumal Jeyaparthasarathy, D'souza Avin, Miriyala Amarnath, Sharma Bhavna, Gopal Ganesh
ITC Limited - Corporate Division, Life Sciences & Technology Centre, #3, 1st Main, Peenya Industrial Area, Phase I, Bangalore, 560 058, India.
ITC Limited - Foods Division, ITC Green Centre, No. 18 Banaswadi, Main Road, Maruthiseva Nagar, Bangalore, 560 005, India.
J Tradit Complement Med. 2022 Feb 2;12(5):455-465. doi: 10.1016/j.jtcme.2022.01.007. eCollection 2022 Sep.
The immune system functions to protect the host from a broad array of infectious diseases. Here, we evaluated the immunomodulatory effects of green coffee extract (GCE), and conducted a double-blinded, randomized and placebo-controlled trial among apparently healthy individuals.
We determined the levels and functions of inflammatory and immune markers viz., phospho-NF-κB p65 ser536, chemotaxis, phagocytosis, TH1/TH2 cytokines and IgG production. We also evaluated several immunological markers such as total leukocyte counts, differential leukocyte counts, NK cell activity, CD4/CD8 ratio, serum immunoglobulin, C-reactive protein (CRP) and pro-inflammatory cytokines (IL-6 and TNF-α).
GCE significantly inhibited LPS-induced NF-κB p65 ser536 phosphorylation, MCP-1-induced chemotaxis and significantly enhanced phagocytosis and IgG production. In addition, GCE modulated PMA/PHA-induced TH1/TH2 cytokine production. Clinical investigations suggested that the expression of CD56 and CD16 was markedly augmented on NK cells following GCE treatment. GCE significantly enhanced IgA production before and after influenza vaccination. Similarly, IL-6, TNF-α and CRP levels were significantly inhibited by GCE. Together, GCE confers several salubrious immunomodulatory effects at different levels attributing to optimal functioning of immune responses in the host.
Cell biology, Clinical study, Clinical Trial.
免疫系统的功能是保护宿主免受多种传染病的侵害。在此，我们评估了绿咖啡提取物（GCE）的免疫调节作用，并在看似健康的个体中进行了一项双盲、随机和安慰剂对照试验。
我们测定了炎症和免疫标志物的水平及功能，即磷酸化核因子κB p65丝氨酸536、趋化作用、吞噬作用、TH1/TH2细胞因子和IgG产生。我们还评估了几种免疫标志物，如白细胞总数、白细胞分类计数、NK细胞活性、CD4/CD8比值、血清免疫球蛋白、C反应蛋白（CRP）和促炎细胞因子（IL-6和TNF-α）。
GCE显著抑制脂多糖诱导的NF-κB p65丝氨酸536磷酸化、单核细胞趋化蛋白-1诱导的趋化作用，并显著增强吞噬作用和IgG产生。此外，GCE调节佛波酯/植物血凝素诱导的TH1/TH2细胞因子产生。临床研究表明，GCE治疗后NK细胞上CD56和CD16的表达明显增加。GCE在流感疫苗接种前后均显著增强IgA产生。同样，GCE显著抑制IL-6、TNF-α和CRP水平。总之，GCE在不同水平上具有多种有益的免疫调节作用，有助于宿主免疫反应的最佳发挥。
细胞生物学、临床研究、临床试验。
