一种精确测量人血浆中内源性血管紧张素I、II和III的灵敏方法。

A sensitive method for precise measurement of endogenous angiotensins I, II&III in human plasma.

作者信息

Kawamura M, Yoshida K, Akabane S, Matsushima Y, Kawano Y, Kojima S, Takahashi N, Shimamoto K, Ito K, Omae T

出版信息

Clin Exp Hypertens A. 1987;9(2-3):687-91. doi: 10.3109/10641968709164243.

DOI:10.3109/10641968709164243

Abstract

We measured endogenous angiotensins (ANGs) I, II&III using a system of extraction by Sep-Pak column followed by high performance liquid chromatography (HPLC) combined with radioimmunoassay (RIA). An excellent separation of ANGs was obtained by HPLC. The recovery of ANGs I, II&III was 80-84%, when these authentic peptides were added to 6 ml of plasma. The coefficient of variation of the ANGs was 0.04-0.09 for intra-assay and 0.08-0.13 for inter-assay, thereby indicating a good reproducibility. Plasma ANGs I, II&III measured by this method in 5 normal volunteers were 51,4.5 and 1.2 pg/ml. In the presence of captopril, ANGs II&III decreased by 84% and 77%, respectively, while ANG I increased 5.1 times. This method is therefore useful to assess the precise levels of plasma ANGs.

摘要

我们采用Sep - Pak柱萃取系统，随后结合高效液相色谱（HPLC）与放射免疫分析（RIA）来测量内源性血管紧张素（ANGs）I、II和III。通过HPLC实现了ANGs的良好分离。当将这些标准肽添加到6毫升血浆中时，ANGs I、II和III的回收率为80 - 84%。ANGs的批内变异系数为0.04 - 0.09，批间变异系数为0.08 - 0.13，表明具有良好的重复性。用该方法测量5名正常志愿者的血浆ANGs I、II和III分别为51、4.5和1.2皮克/毫升。在卡托普利存在的情况下，ANGs II和III分别下降了84%和77%，而ANG I增加了5.1倍。因此，该方法有助于评估血浆ANGs的精确水平。